Yampolskaya, Daria S., Kopylova, Galina V., Shchepkin, Daniil V., Nabiev, Salavat R., Nikitina, Larisa V., Walklate, Jonathan, Ziganshin, Rustam H., Bershitsky, Sergey Y., Geeves, Michael A., Matyushenko, Alexander M., and others. (2022) Pseudo-phosphorylation of essential light chains affects the functioning of skeletal muscle myosin. Biophysical Chemistry, 292 . Article Number 106936. ISSN 0301-4622. E-ISSN 1873-4200. (doi:10.1016/j.bpc.2022.106936) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:99064)
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Official URL: https://doi.org/10.1016/j.bpc.2022.106936 |
Abstract
The work aimed to investigate how the phosphorylation of the myosin essential light chain of fast skeletal myosin (LC1) affects the functional properties of the myosin molecule. Using mass-spectrometry, we revealed phosphorylated peptides of LC1 in myosin from different fast skeletal muscles. Mutations S193D and T65D that mimic natural phosphorylation of LC1 were produced, and their effects on functional properties of the entire myosin molecule and isolated myosin head (S1) were studied. We have shown that T65D mutation drastically decreased the sliding velocity of thin filaments in an in vitro motility assay and strongly increased the duration of actin-myosin interaction in optical trap experiments. These effects of T65D mutation in LC1 observed only with the whole myosin but not with S1 were prevented by double T65D/S193D mutation. The T65D and T65D/S193D mutations increased actin-activated ATPase activity of S1 and decreased ADP affinity for the actin-S1 complex. The results indicate that pseudo-phosphorylation of LC1 differently affects the properties of the whole myosin molecule and its isolated head. Also, the results show that phosphorylation of LC1 of skeletal myosin could be one more mechanism of regulation of actin-myosin interaction that needs further investigation.
Item Type: | Article |
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DOI/Identification number: | 10.1016/j.bpc.2022.106936 |
Uncontrolled keywords: | Myosin, Essential light chain, Pseudo-phosphorylation, Actin-myosin interaction, Mechanism of muscle contraction |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Funders: | University of Kent (https://ror.org/00xkeyj56) |
SWORD Depositor: | JISC Publications Router |
Depositing User: | JISC Publications Router |
Date Deposited: | 25 Jan 2023 13:46 UTC |
Last Modified: | 06 Feb 2023 09:15 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/99064 (The current URI for this page, for reference purposes) |
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