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Enhanced resolution optoacoustic microscopy using a picosecond high repetition rate Q-switched microchip laser

Nteroli, Gianni, Messa, Giulia, Dasa, Manoj K., Penttinen, Antti, Härkönen, Antti, Guina, Mircea, Podoleanu, Adrian G.H., Koutsikou, Stella, Bradu, Adrian (2022) Enhanced resolution optoacoustic microscopy using a picosecond high repetition rate Q-switched microchip laser. Journal of Biomedical Optics, 27 (11). Article Number 110501. ISSN 1083-3668. E-ISSN 1560-2281. (doi:10.1117/1.JBO.27.11.110501) (KAR id:98484)

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Conventional optoacoustic microscopy (OAM) instruments have at their core a nanosecond pulse duration laser. If lasers with a shorter pulse duration are used, broader, higher frequency ultrasound waves are expected to be generated and as a result, the axial resolution of the instrument is improved. Here, we exploit the advantage offered by a picosecond duration pulse laser to enhance the axial resolution of an OAM instrument. In comparison to an instrument equipped with a 2-ns pulse duration laser, an improvement in the axial resolution of 50% is experimentally demonstrated by using excitation pulses of only 85 ps. To illustrate the capability of the instrument to generate high-quality optoacoustic images, en-face, in-vivo images of the brain of Xenopus laevis tadpole are presented with a lateral resolution of 3.8 μm throughout the entire axial imaging range.

Item Type: Article
DOI/Identification number: 10.1117/1.JBO.27.11.110501
Additional information: For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.
Uncontrolled keywords: photoacoustics, picoseconds, lasers
Subjects: Q Science > QC Physics > QC355 Optics
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Funders: Academy of Medical Sciences (
Biotechnology and Biological Sciences Research Council (
Depositing User: Adrian Bradu
Date Deposited: 29 Nov 2022 09:36 UTC
Last Modified: 18 Jul 2023 15:12 UTC
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Nteroli, Gianni:
Dasa, Manoj K.:
Podoleanu, Adrian G.H.:
Koutsikou, Stella:
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