Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS

Garcia, Juliana, Oliveira, Ana, de Pinho, Paula Guedes, Freitas, Victor, Carvalho, Alexandra, Baptista, Paula, Pereira, Eric, de Lourdes Bastos, Maria, Carvalho, Félix (2015) Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS. Mycologia, 107 (4). pp. 679-687. ISSN 0027-5514. (doi:10.3852/14-253) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:98364)

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Abstract

Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. α-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.

Item Type:	Article
DOI/Identification number:	10.3852/14-253
Uncontrolled keywords:	α-amanitin, Amanita phalloides, mass spectrometry, reversed-phase high performance liquid chromatography, toxins
Subjects:	R Medicine
Divisions:	Divisions > Division of Natural Sciences > Kent and Medway Medical School
Depositing User:	Ana Oliveira
Date Deposited:	26 Nov 2022 19:51 UTC
Last Modified:	05 Nov 2024 13:03 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/98364 (The current URI for this page, for reference purposes)

University of Kent Author Information

Oliveira, Ana.

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