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Streptomyces cell-free systems for natural product discovery and engineering

Moore, Simon J., Lai, Hung-En, Li, Jian, Freemont, Paul S (2022) Streptomyces cell-free systems for natural product discovery and engineering. Natural Product Reports, . ISSN 0265-0568. E-ISSN 1460-4752. (doi:10.1039/d2np00057a) (KAR id:98193)


Streptomyces bacteria are a major microbial source of natural products, which are encoded within so-called biosynthetic gene clusters (BGCs). This highlight discusses the emergence of native Streptomyces cell-free systems as a new tool to accelerate the study of the fundamental chemistry and biology of natural product biosynthesis from these bacteria. Cell-free systems provide a prototyping platform to study plug-and-play reactions in microscale reactions. So far, Streptomyces cell-free systems have been used to rapidly characterise gene expression regulation, access secondary metabolite biosynthetic enzymes, and catalyse cell-free transcription, translation, and biosynthesis of example natural products. With further progress, we anticipate the development of more complex systems to complement existing experimental tools for the discovery and engineering of natural product biosynthesis from Streptomyces and related high G + C (%) bacteria.

Item Type: Article
DOI/Identification number: 10.1039/d2np00057a
Additional information: For the purpose of open access, the author(s) has applied a Creative Commons Attribution (CC BY) licence to any Author Accepted Manuscript version arising.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Wellcome Trust (
Leverhulme Trust (
Royal Society (
SWORD Depositor: JISC Publications Router
Depositing User: JISC Publications Router
Date Deposited: 21 Nov 2022 11:29 UTC
Last Modified: 12 Dec 2023 12:56 UTC
Resource URI: (The current URI for this page, for reference purposes)

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