Skip to main content

Transcriptional profiling of Pseudomonas aeruginosa mature single and dual species biofilms in response to meropenem

Alam, Farhana, Blair, Jessica M A, Hall, Rebecca A. (2023) Transcriptional profiling of Pseudomonas aeruginosa mature single and dual species biofilms in response to meropenem. Microbiology, 169 (1). ISSN 1350-0872. E-ISSN 1465-2080. (doi:10.1099/mic.0.001271) (KAR id:97904)

PDF Publisher pdf
Language: English


Download (4MB) Preview
[thumbnail of Hall_Transcriptional profiling_1.pdf]
Preview
This file may not be suitable for users of assistive technology.
Request an accessible format
PDF Author's Accepted Manuscript
Language: English


Download (610kB) Preview
[thumbnail of Transcriptional profiling of Pseudomonas aeruginosa.pdf]
Preview
This file may not be suitable for users of assistive technology.
Request an accessible format
XML Word Processing Document (DOCX) Author's Accepted Manuscript
Language: English

Restricted to Repository staff only

Contact us about this Publication
[thumbnail of version5-clean.docx]
Official URL:
https://doi.org/10.1099/mic.0.001271

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen frequently isolated from chronic infections of the Cystic Fibrosis lung and burn wounds, and is a major cause of antimicrobial resistant nosocomial infections. P. aeruginosa is frequently co-isolated with the opportunistic fungal pathogen C. albicans, with the presence of C. albicans in dual species biofilms promoting tolerance to meropenem. Here, transcription profiling of mature P. aeruginosa single or dual species biofilms was carried out to understand the molecular mechanism(s) by which C. albicans enhances meropenem tolerance. C. albicans appeared to have a mild impact on the transcriptome of P. aeruginosa mature biofilms, with most differentially regulated genes being involved in interkingdom interactions (i.e. quorum sensing, and phenazine biosynthesis). The addition of meropenem to mature single or dual species biofilms resulted in a significant bacterial transcriptional response, including the induction of the beta-lactamase, ampC, genes involved in biofilm formation. P. aeruginosa, elicited a similar transcriptional response to meropenem in the presence of C. albicans, but C. albicans promoted the expression of additional efflux pumps, which could play roles in increasing the tolerance of P. aeruginosa to meropenem.

Item Type: Article
DOI/Identification number: 10.1099/mic.0.001271
Uncontrolled keywords: Candida albicans, Pseudomonas aeruginosa, meropenem, transcriptional profiling, dual-species biofilms
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (https://ror.org/00cwqg982)
Medical Research Council (https://ror.org/03x94j517)
Wellcome Trust (https://ror.org/029chgv08)
Depositing User: Becky Hall
Date Deposited: 10 Nov 2022 09:11 UTC
Last Modified: 24 Jan 2023 09:00 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/97904 (The current URI for this page, for reference purposes)
Hall, Rebecca A.: https://orcid.org/0000-0002-4908-8168
  • Depositors only (login required):

Downloads

Downloads per month over past year