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A mini-review: environmental and metabolic factors affecting aminoglycoside efficacy

Webster, Calum M., Shepherd, Mark (2022) A mini-review: environmental and metabolic factors affecting aminoglycoside efficacy. World Journal of Microbiology and Biotechnology, 39 (1). Article Number 7. E-ISSN 1573-0972. (doi:10.1007/s11274-022-03445-8) (KAR id:97709)


Following the discovery of streptomycin from Streptomyces griseus in the 1940s by Selman Waksman and colleagues, aminoglycosides were first used to treat tuberculosis and then numerous derivatives have since been used to combat a wide variety of bacterial infections. These bactericidal antibiotics were used as first-line treatments for several decades but were largely replaced by ß-lactams and fluoroquinolones in the 1980s, although widespread emergence of antibiotic-resistance has led to renewed interest in aminoglycosides. The primary site of action for aminoglycosides is the 30 S ribosomal subunit where they disrupt protein translation, which contributes to widespread cellular damage through a number of secondary effects including rapid uptake of aminoglycosides via elevated proton-motive force (PMF), membrane damage and breakdown, oxidative stress, and hyperpolarisation of the membrane. Several factors associated with aminoglycoside entry have been shown to impact upon bacterial killing, and more recent work has revealed a complex relationship between metabolic states and the efficacy of different aminoglycosides. Hence, it is imperative to consider the environmental conditions and bacterial physiology and how this can impact upon aminoglycoside entry and potency. This mini-review seeks to discuss recent advances in this area and how this might affect the future use of aminoglycosides.

Item Type: Article
DOI/Identification number: 10.1007/s11274-022-03445-8
Projects: RAPID Group
Uncontrolled keywords: reactive oxygen species, aminoglycoside, proton motive force, bacteria, streptomyces griseus, aminoglycosides, anti-bacterial agents, drug resistance, microbial
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: University of Kent (
Depositing User: Mark Shepherd
Date Deposited: 31 Oct 2022 14:48 UTC
Last Modified: 19 Dec 2022 10:35 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Webster, Calum M..

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Shepherd, Mark.

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