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An analysis of respiratory function and mitochondrial morphology in Candida albicans

Duvenage, Lucian, Daniel, Pentland R., Munro, Carol, Gourlay, Campbell W. (2019) An analysis of respiratory function and mitochondrial morphology in Candida albicans. BioRXiV, . (Submitted) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:97699)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL:
https://doi.org/10.1101/697516

Abstract

Respiratory function and mitochondrial dynamics have been well characterised in a number of cell types, including the model yeast Saccharomyces cerevisiae, but remain under-researched in fungal pathogens such as Candida albicans. An understanding of mitochondrial activity and morphology is important if we are to understand the role that this organelle plays in adaption and response to stress. Here we examine the respiratory profiles of several prominent pathogenic Candida species and present a useful GFP probe for the study of mitochondrial morphology. We examine mitochondrial morphology under a variety of conditions that Candida species may encounter within the host, such as acidic pH, respiratory and oxidative stress. The GFP probe also allowed for the visualisation of mitochondria during hyphal development, during growth following macrophage engulfment and distribution within biofilms. These data demonstrate that the mitochondrial network of C. albicans is highly responsive to both environmental conditions and developmental cues, suggesting important roles for this organelle in environmental adaption.

Item Type: Article
Subjects: Q Science
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Wellcome Trust (https://ror.org/029chgv08)
Depositing User: Campbell Gourlay
Date Deposited: 30 Oct 2022 16:02 UTC
Last Modified: 01 Nov 2022 10:55 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/97699 (The current URI for this page, for reference purposes)
Gourlay, Campbell W.: https://orcid.org/0000-0002-2373-6788
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