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DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins

Charman, Robert J. and Kad, Neil M. (2022) DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins. In: Chromosome Architecture : Methods and Protocols. Methods in Molecular Biology . Springer, pp. 129-143. ISBN 978-1-07-162220-9. E-ISBN 978-1-07-162221-6. (doi:10.1007/978-1-0716-2221-6_10) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:95698)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL:
https://doi.org/10.1007/978-1-0716-2221-6_10

Abstract

Many protein interactions with DNA are reliant on the presence of specific DNA sequences, adducts, or structures. In bulk-phase experiments such DNA features are facile to include in a study. For single-molecule imaging this can be more difficult, because the constraints of the assay limit the variety of adducts that can be used. Surface-immobilized DNA provides an ideal compromise, and the use of interferometric scattering microscopy allows for high-speed imaging of these interactions. Furthermore, this technique offers the ability to identify binder stoichiometry and the composition of protein complexes. Its implementation is relatively simple; however data analysis and deconvolution are more challenging. In this chapter we examine how this technique is implemented and reveal software that can be used to deconvolute the images. Altogether, we hope to make this technique more accessible for studying specific DNA-protein interactions on tailored substrates.

Item Type: Book section
DOI/Identification number: 10.1007/978-1-0716-2221-6_10
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Neil Kad
Date Deposited: 07 Jul 2022 09:58 UTC
Last Modified: 08 Jul 2022 08:28 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/95698 (The current URI for this page, for reference purposes)

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