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Two octaves spanning photoacoustic microscopy

Nteroli, Gianni, Dasa, Manoj Kumar, Messa, Giulia, Koutsikou, Stella, Bondu, Magalie, Moselund, Peter Morten, Markos, Christos, Bang, Ole, Podoleanu, Adrian G.H., Bradu, Adrian and others. (2022) Two octaves spanning photoacoustic microscopy. Scientific Reports, 12 . Article Number 10590. ISSN 2045-2322. (doi:10.1038/s41598-022-14869-5) (KAR id:95550)

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In this study, for the first time, a Photoacoustic Microscopy instrument driven by a single optical source operating over a wide spectral range (475–2400 nm), covering slightly more than two octaves is demonstrated. Xenopus laevis tadpoles were imaged in vivo using the whole spectral range of 2000 nm of a supercontinuum optical source, and a novel technique of mapping absorbers is also demonstrated, based on the supposition that only one chromophore contributes to the photoacoustic signal of each individual voxel in the 3D photoacoustic image. By using a narrow spectral window (of 25 nm bandwidth) within the broad spectrum of the supercontinuum source at a time, in vivo hyper-spectral Photoacoustic images of tadpoles are obtained. By post-processing pairs of images obtained using different spectral windows, maps of five endogenous contrast agents (hemoglobin, melanin, collagen, glucose and lipids) are produced.

Item Type: Article
DOI/Identification number: 10.1038/s41598-022-14869-5
Subjects: Q Science > QC Physics > QC355 Optics
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Adrian Bradu
Date Deposited: 28 Jun 2022 16:45 UTC
Last Modified: 12 Jan 2024 07:28 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

Nteroli, Gianni.

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Messa, Giulia.

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Koutsikou, Stella.

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Bondu, Magalie.

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Podoleanu, Adrian G.H..

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Bradu, Adrian.

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