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Factors affecting de novo formation of a yeast prion

Stojanovski, Klement (2012) Factors affecting de novo formation of a yeast prion. Doctor of Philosophy (PhD) thesis, University of Kent. (doi:10.22024/UniKent/01.02.94678) (KAR id:94678)

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Prions are aggregates of misfolded proteins that have acquired an amyloid-like structure and ability to propagate through recruitment of new proteins. [PSI+], a prion form of eukaryotic release factor Sup35 (eRF1) is widely used as a model for research on prion formation and propagation and in this study [PSI+] is used to explore an effect of three previously identified proteins on de novo prion formation. One mechanism proposed to affect prion formation is direct interaction of Sup35p with its binding partners and search for proteins that interact with Sup35p identified Ppq 1 p, a putative Ser/Thr protein phosphatase (M.F. Tuite and T. von der Haar). Another approach was to identify proteins that function to protect translational apparatus from environmental and . endogenous oxidative damage. and this approach identified two ribosome associated peroxiredoxins, Tsa1 p and Tsa2p (T. Sideri and C.M. Grant). The data presented here shows that the deletion of PPQ1 gene greatly increases the rate of de novo formation of [PSI+] but the mechanism by which loss of Ppq1 p affects [PSI+] formation is not known. Analysis of the distribution of fluorescently-tagged Ppq 1 P showed that the protein co-localises with mitochondria. A further line of evidence linking Ppq 1 P to mitochondria was an observed reduction in respiratory capacity of a ppq1 Δ strain. That exposure to environmental sources of oxidative stress promotes [PSI+] prion formation was previously reported (Tyedmers et al., 2008). Results presented here show that an endogenous source of oxidative stress, brought about by deleting the ribosomally- associated peroxiredoxins (Prx) encoded by genes TSA 1/2 (Trotter et al., 2008; Sideri et al., 2010), also increases the rate of de novo [PSI+]formation. This result provides a direct link between oxidative stress and the eukaryotic release factor Sup35p.

Item Type: Thesis (Doctor of Philosophy (PhD))
Thesis advisor: Tuite, Mick
DOI/Identification number: 10.22024/UniKent/01.02.94678
Additional information: This thesis has been digitised by EThOS, the British Library digitisation service, for purposes of preservation and dissemination. It was uploaded to KAR on 25 April 2022 in order to hold its content and record within University of Kent systems. It is available Open Access using a Creative Commons Attribution, Non-commercial, No Derivatives ( licence so that the thesis and its author, can benefit from opportunities for increased readership and citation. This was done in line with University of Kent policies ( If you feel that your rights are compromised by open access to this thesis, or if you would like more information about its availability, please contact us at and we will seriously consider your claim under the terms of our Take-Down Policy (
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
SWORD Depositor: SWORD Copy
Depositing User: SWORD Copy
Date Deposited: 02 Sep 2022 15:32 UTC
Last Modified: 02 Sep 2022 15:32 UTC
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