Skip to main content

A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation

Josse, Eve-Marie, Simkin, Andrew J., Gaffé, Joël, Labouré, Anne-Marie, Kuntz, Marcel, Carol, Pierre (2000) A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiology, 123 (4). pp. 1427-1436. ISSN 0032-0889. E-ISSN 1532-2548. (doi:10.1104/pp.123.4.1427) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:93897)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL
https://doi.org/10.1104/pp.123.4.1427

Abstract

The Arabidopsis IMMUTANS gene encodes a plastid homolog of the mitochondrial alternative oxidase, which is associated with phytoene desaturation. Upon expression in Escherichia coli, this protein confers a detectable cyanide-resistant electron transport to isolated membranes. In this assay this activity is sensitive to n-propyl-gallate, an inhibitor of the alternative oxidase. This protein appears to be a plastid terminal oxidase (PTOX) that is functionally equivalent to a quinol:oxygen oxidoreductase. This protein was immunodetected in achlorophyllous pepper (Capsicum annuum) chromoplast membranes, and a corresponding cDNA was cloned from pepper and tomato (Lycopersicum esculentum) fruits. Genomic analysis suggests the presence of a single gene in these organisms, the expression of which parallels phytoene desaturase and ζ-carotene desaturase gene expression during fruit ripening. Furthermore, thisPTOX gene is impaired in the tomato ghostmutant, which accumulates phytoene in leaves and fruits. These data show that PTOX also participates in carotenoid desaturation in chromoplasts in addition to its role during early chloroplast development.

Item Type: Article
DOI/Identification number: 10.1104/pp.123.4.1427
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Andy Simkin
Date Deposited: 05 May 2022 18:33 UTC
Last Modified: 06 May 2022 08:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93897 (The current URI for this page, for reference purposes)
Simkin, Andrew J.: https://orcid.org/0000-0001-5056-1306
  • Depositors only (login required):