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Circadian Regulation of the PhCCD1 Carotenoid Cleavage Dioxygenase Controls Emission of β-Ionone, a Fragrance Volatile of Petunia Flowers

Simkin, Andrew J., Underwood, Beverly A., Auldridge, Michele, Loucas, Holly M., Shibuya, Kenichi, Schmelz, Eric, Clark, David G., Klee, Harry J. (2004) Circadian Regulation of the PhCCD1 Carotenoid Cleavage Dioxygenase Controls Emission of β-Ionone, a Fragrance Volatile of Petunia Flowers. Plant Physiology, 136 (3). pp. 3504-3514. ISSN 0032-0889. E-ISSN 1532-2548. (doi:10.1104/pp.104.049718) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:93894)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL
https://doi.org/10.1104/pp.104.049718

Abstract

Carotenoids are thought to be the precursors of terpenoid volatile compounds that contribute to flavor and aroma. One such volatile, β-ionone, is important to fragrance in many flowers, including petunia (Petunia hybrida). However, little is known about the factors regulating its synthesis in vivo. The petunia genome contains a gene encoding a 9,10(9′,10′) carotenoid cleavage dioxygenase, PhCCD1. The PhCCD1 is 94% identical to LeCCD1A, an enzyme responsible for formation of β-ionone in tomato (Lycopersicon esculentum; Simkin AJ, Schwartz SH, Auldridge M, Taylor MG, Klee HJ [2004] Plant J [in press]). Reduction of PhCCD1 transcript levels in transgenic plants led to a 58% to 76% decrease in β-ionone synthesis in the corollas of selected petunia lines, indicating a significant role for this enzyme in volatile synthesis. Quantitative reverse transcription-PCR analysis revealed that PhCCD1 is highly expressed in corollas and leaves, where it constitutes approximately 0.04% and 0.02% of total RNA, respectively. PhCCD1 is light-inducible and exhibits a circadian rhythm in both leaves and flowers. β-Ionone emission by flowers occurred principally during daylight hours, paralleling PhCCD1 expression in corollas. The results indicate that PhCCD1 activity and β-ionone emission are likely regulated at the level of transcript.

Item Type: Article
DOI/Identification number: 10.1104/pp.104.049718
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Andy Simkin
Date Deposited: 05 May 2022 18:34 UTC
Last Modified: 06 May 2022 08:59 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93894 (The current URI for this page, for reference purposes)
Simkin, Andrew J.: https://orcid.org/0000-0001-5056-1306
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