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Ethylene-Regulated Floral Volatile Synthesis in Petunia Corollas

Underwood, Beverly A., Tieman, Denise M., Shibuya, Kenichi, Dexter, Richard J., Loucas, Holly M., Simkin, Andrew J., Sims, Charles A., Schmelz, Eric A., Klee, Harry J., Clark, David G. and others. (2005) Ethylene-Regulated Floral Volatile Synthesis in Petunia Corollas. Plant Physiology, 138 (1). pp. 255-266. ISSN 0032-0889. E-ISSN 1532-2548. (doi:10.1104/pp.104.051144) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:93890)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL
https://doi.org/10.1104/pp.104.051144

Abstract

In many flowering plants, such as petunia (Petunia × hybrida), ethylene produced in floral organs after pollination elicits a series of physiological and biochemical events, ultimately leading to senescence of petals and successful fertilization. Here, we demonstrate, using transgenic ethylene insensitive (44568) and Mitchell Diploid petunias, that multiple components of emission of volatile organic compounds (VOCs) are regulated by ethylene. Expression of benzoic acid/salicylic acid carboxyl methyltransferase (PhBSMT1 and 2) mRNA is temporally and spatially down-regulated in floral organs in a manner consistent with current models for postpollination ethylene synthesis in petunia corollas. Emission of methylbenzoate and other VOCs after pollination and exogenous ethylene treatment parallels a reduction in PhBSMT1 and 2 mRNA levels. Under cyclic light conditions (day/night), PhBSMT mRNA levels are rhythmic and precede emission of methylbenzoate by approximately 6 h. When shifted into constant dark or light conditions, PhBSMT mRNA levels and subsequent methylbenzoate emission correspondingly decrease or increase to minimum or maximum levels observed during normal conditions, thus suggesting that light may be a more critical influence on cyclic emission of methylbenzoate than a circadian clock. Transgenic PhBSMT RNAi flowers with reduced PhBSMT mRNA levels show a 75% to 99% decrease in methylbenzoate emission, with minimal changes in other petunia VOCs. These results implicate PhBSMT1 and 2 as genes responsible for synthesis of methylbenzoate in petunia.

Item Type: Article
DOI/Identification number: 10.1104/pp.104.051144
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Andy Simkin
Date Deposited: 05 May 2022 18:49 UTC
Last Modified: 06 May 2022 09:34 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93890 (The current URI for this page, for reference purposes)
Simkin, Andrew J.: https://orcid.org/0000-0001-5056-1306
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