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Localization and Functional Characterization of the Alternative Oxidase in Naegleria

Cantoni, Diego, Osborne, Ashley, Taib, Najwa, Thompson, Gary S., Martín‐Escolano, Rubén, Kazana, Eleanna, Edrich, Elizabeth, Brown, Ian R., Gribaldo, Simonetta, Gourlay, Campbell W., and others. (2022) Localization and Functional Characterization of the Alternative Oxidase in Naegleria. Journal of Eukaryotic Microbiology, . ISSN 1066-5234. (doi:10.1111/jeu.12908) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:93807)

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Abstract

The alternative oxidase (AOX) is a protein involved in supporting enzymatic reactions of the Krebs cycle in instances when the canonical (cytochrome-mediated) respiratory chain has been inhibited, while allowing for the maintenance of cell growth and necessary metabolic processes for survival. Among eukaryotes, alternative oxidases have dispersed distribution and are found in plants, fungi, and protists, including Naegleria ssp. Naegleria species are free-living unicellular amoeboflagellates, and include the pathogenic species of N. fowleri, the so-called brain eating amoeba. Using a multidisciplinary approach, we aimed to understand the evolution, localization and function of AOX and the role that plays in Naegleria’s biology. Our analyses suggest that AOX was present in last common ancestor of the genus and structure prediction showed that all functional residues are also present in Naegleria species. Using cellular and biochemical techniques, we also functionally characterize N. gruberi’s AOX in its mitochondria and we demonstrate that its inactivation affects its proliferation. Consequently, we discuss the benefits of the presence of this protein in Naegleria species, along with its potential pathogenicity role in N. fowleri. We predict that our findings will spearhead new explorations to understand the cell biology, metabolism and evolution of Naegleria and other free-living relatives.

Item Type: Article
DOI/Identification number: 10.1111/jeu.12908
Uncontrolled keywords: AOX, Naegleria, mitochondria
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Campbell Gourlay
Date Deposited: 01 Apr 2022 08:21 UTC
Last Modified: 04 Apr 2022 09:54 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/93807 (The current URI for this page, for reference purposes)
Thompson, Gary S.: https://orcid.org/0000-0001-9399-7636
Gourlay, Campbell W.: https://orcid.org/0000-0002-2373-6788
Tsaousis, Anastasios D.: https://orcid.org/0000-0002-5424-1905
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