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Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking

Marques, Manuel, Hughes, Michael, Fernandez Uceda, Adrian, Gelikonov, Grigory, Bradu, Adrian, Podoleanu, Adrian (2022) Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking. Biomedical Optics Express, 13 (2). pp. 761-776. E-ISSN 2156-7085. (doi:10.1364/BOE.444170) (KAR id:92678)

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Abstract

Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D imaging in vivo, and can be combined with widefield fluorescence imaging by use of a double-clad fiber. However, it is technically challenging to build a high-performance miniaturized 2D scanning system with a large field-of-view. In this paper we demonstrate how a 1D scanning probe, which produces cross-sectional OCT images (B-scans) and 1D fluorescence T-scans, can be transformed into a 2D scanning probe by manual scanning along the second axis. OCT volumes are assembled from the B-scans using speckle decorrelation measurements to estimate the out-of-plane motion along the manual scan direction. Motion within the plane of the B-scans is corrected using image registration by normalized cross correlation. En-face OCT slices and fluorescence images, corrected for probe motion in 3D, can be displayed in real-time during the scan. For a B-scan frame rate of 250 Hz, and an OCT lateral resolution of approximately 20µ m, the approach can handle out-of-plane motion at speeds of up to 4 mm/s.

Item Type: Article
DOI/Identification number: 10.1364/BOE.444170
Subjects: Q Science > QC Physics > QC355 Optics
Q Science > QC Physics > QC411 Interference
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Manuel Marques
Date Deposited: 13 Jan 2022 08:36 UTC
Last Modified: 22 Mar 2022 14:58 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/92678 (The current URI for this page, for reference purposes)
Marques, Manuel: https://orcid.org/0000-0002-7509-0704
Hughes, Michael: https://orcid.org/0000-0001-5301-2492
Bradu, Adrian: https://orcid.org/0000-0002-6890-1599
Podoleanu, Adrian: https://orcid.org/0000-0002-4899-9656
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