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Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers

Betts, Emma L., Newton, Jamie M., Robinson, Gary K., Sarzhanov, Fakhriddin, Jinatham, Vasana, Kim, Moon-Ju, Popluechai, Siam, Dogruman-Al, Funda, Won, Eun Jeong, Gentekaki, Eleni, and others. (2021) Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers. Metabolites, 11 (12). Article Number 883. ISSN 2218-1989. (doi:10.3390/metabo11120883) (KAR id:92245)

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Abstract

(1) Background: Blastocystis is an obligate anaerobic microbial eukaryote that frequently inhabits the gastrointestinal tract. Despite this prevalence, very little is known about the extent of its genet-ic diversity, pathogenicity, and interaction with the rest of the microbiome and its host. Although the organism is morphologically static, it has no less than 28 genetically distinct subtypes (STs). Reports on the pathogenicity of Blastocystis are conflicting. The association between Blastocystis and intestinal bacterial communities is being increasingly explored. Nonetheless, similar inves-tigations extending to the metabolome are non-existent. (2) Methods: Using established NMR metabolomics protocols in 149 faecal samples from individuals from South Korea (n=38), Thai-land (n=44) and Turkey (n=69), we have provided a snapshot of the core metabolic compounds present in human stools with (B+) and without (B-) Blastocystis. Samples included hosts with gastrointestinal symptoms and asymptomatics. (3) Results: A total of nine, 62 and 98 significant metabolites were associated with Blastocystis carriage in the South Korean, Thai and Turkish sample sets respectively, with a number of metabolites increased in colonized groups. The meta-bolic profiles of B+ and B- samples from all countries were distinct and grouped separately in the partial least squares-discriminant analysis (PLS-DA). Typical inflammation-related metabolites negatively associated with Blastocystis positive samples. (4) Conclusions: This data will assist in directing future studies underlying involvement of Blastocystis in physiological processes of both the gut microbiome and the host. Future studies using metabolome and microbiome data along with host physiology and immune responses information will contribute significantly towards elucidating the role of Blastocystis in health and disease.

Item Type: Article
DOI/Identification number: 10.3390/metabo11120883
Projects: [UNSPECIFIED] Exploring the anaerobic adaptations of the mitochondrion-related organelles of Blastocystis
Uncontrolled keywords: Amino acids; Blastocystis; inflammation; metabolomics; metabolite profiles; stool; NMR
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: [39] BBSRC
Depositing User: Anastasios Tsaousis
Date Deposited: 09 Dec 2021 13:45 UTC
Last Modified: 17 Mar 2022 12:47 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/92245 (The current URI for this page, for reference purposes)
Robinson, Gary K.: https://orcid.org/0000-0003-2660-7778
Tsaousis, Anastasios D.: https://orcid.org/0000-0002-5424-1905
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