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Worms With a Single Functional Sensory Cilium Generate Proper Neuron-Specific Behavioral Output

Senti, Gabriele, Ezcurra, Marina, Löbner, Jana, Schafer, William R., Swoboda, Peter (2009) Worms With a Single Functional Sensory Cilium Generate Proper Neuron-Specific Behavioral Output. Genetics, 183 (2). pp. 595-605. ISSN 0016-6731. E-ISSN 1943-2631. (doi:10.1534/genetics.109.105171) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:91259)

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Studying the development and mechanisms of sensory perception is challenging in organisms with

complex neuronal networks. The worm Caenorhabditis elegans possesses a simple neuronal network of 302 neurons that includes 60 ciliated sensory neurons (CSNs) for detecting external sensory input. C. elegans is thus an excellent model in which to study sensory neuron development, function, and behavior. We have generated a genetic rescue system that allows in vivo analyses of isolated CSNs at both cellular and systemic levels. We used the RFX transcription factor DAF-19, a key regulator of ciliogenesis. Mutations in daf-19 result in the complete absence of all sensory cilia and thus of external sensory input. In daf-19 mutants, we used cell-specific rescue of DAF-19 function in selected neurons, thereby generating animals with single, fully functional CSNs. Otherwise and elsewhere these animals are completely devoid of any environmental input through cilia. We demonstrated the rescue of fully functional, single cilia using fluorescent markers, sensory behavioral assays, and calcium imaging. Our technique, functional rescue in single sensory cilia (FRISSC), can thus cell-autonomously and cell-specifically restore the function of single sensory neurons and their ability to respond to sensory input. FRISSC can be adapted to many different CSNs and thus constitutes an excellent tool for studying sensory behaviors, both in single animals and in populations of worms. FRISSC will be very useful for the molecular dissection of sensory perception in CSNs and for the analysis of the developmental aspects of ciliogenesis.

Item Type: Article
DOI/Identification number: 10.1534/genetics.109.105171
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Marina Ezcurra
Date Deposited: 02 Nov 2021 11:55 UTC
Last Modified: 16 Nov 2021 10:27 UTC
Resource URI: (The current URI for this page, for reference purposes)

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