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The Genome of the CTG(Ser1) Yeast Scheffersomyces stipitis Is Plastic

Vega-Estévez, Samuel, Armitage, Andrew, Bates, Helen J., Harrison, Richard J., Buscaino, Alessia (2021) The Genome of the CTG(Ser1) Yeast Scheffersomyces stipitis Is Plastic. mBio, . E-ISSN 2150-7511. (doi:10.1128/mBio.01871-21) (KAR id:90336)

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Microorganisms need to adapt to environmental changes, and genome plasticity can lead to rapid adaptation to hostile environments by increasing genetic diversity. Here, we investigate genome plasticity in the CTG(Ser1) yeast Scheffersomyces stipitis, an organism with an enormous potential for second-generation biofuel production. We demonstrate that S. stipitis has an intrinsically plastic genome and that different S. stipitis isolates have genomes with distinct chromosome organizations. Real-time evolution experiments show that S. stipitis genome plasticity is common and rapid since extensive genomic changes with fitness benefits are detected following in vitro evolution experiments. Hybrid MinION Nanopore and Illumina genome sequencing identify retrotransposons as major drivers of genome diversity. Indeed, the number and position of retrotransposons are different in different S. stipitis isolates, and retrotransposon-rich regions of the genome are sites of chromosome rearrangements. Our findings provide important insights into the adaptation strategies of the CTG(Ser1) yeast clade and have critical implications in the development of second-generation biofuels. These data highlight that genome plasticity is an essential factor for developing sustainable S. stipitis platforms for second-generation biofuels production.

Item Type: Article
DOI/Identification number: 10.1128/mBio.01871-21
Uncontrolled keywords: CTG clade yeast; biofuels; genome plasticity; genomic instability; transposons
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 24 Sep 2021 13:23 UTC
Last Modified: 14 Nov 2022 23:13 UTC
Resource URI: (The current URI for this page, for reference purposes)
Buscaino, Alessia:
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