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Porin 1 Modulates Autophagy in Yeast

Gourlay, Campbell (2021) Porin 1 Modulates Autophagy in Yeast. Cells, . ISSN 2073-4409. (doi:10.3390/cells10092416) (KAR id:90210)

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Official URL
https://www.mdpi.com/2073-4409/10/9/2416

Abstract

Autophagy is a cellular recycling program which efficiently reduces the cellular burden of ageing. Autophagy is characterised by nucleation of isolation membranes, which grow in size and further expand to form autophagosomes, engulfing cellular material to be degraded by fusion with lysosomes (vacuole in yeast). Autophagosomal membranes do not bud from a single cell organelle, but are generated de novo. Several lipid sources for autophagosomal membranes have been identified, but the whole process of their generation is complex and not entirely understood. In this study, we investigated how the mitochondrial outer membrane protein porin 1 (Por1), the yeast orthologue of mammalian voltage-dependent anion channel (VDAC), affects autophagy in yeast. We show that POR1 deficiency reduces the autophagic capacity and leads to changes in vacuole and lipid homeostasis. We further investigated whether limited phosphatidylethanolamine (PE) availability in por1∆ was causative for reduced autophagy by overexpression of the PE-generating phosphatidylserine decarboxylase 1 (Psd1). Altogether, our results show that POR1 deficiency is associated with reduced autophagy, which can be circumvented by additional PSD1 overexpression. This suggests a role for Por1 in Psd1-mediated autophagy regulation.

Item Type: Article
DOI/Identification number: 10.3390/cells10092416
Subjects: Q Science > Q Science (General)
Divisions: Divisions > Division of Natural Sciences > Biosciences
Signature Themes: Future Human
Depositing User: Campbell Gourlay
Date Deposited: 14 Sep 2021 14:13 UTC
Last Modified: 15 Sep 2021 10:42 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/90210 (The current URI for this page, for reference purposes)
Gourlay, Campbell: https://orcid.org/0000-0002-2373-6788
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