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A novel Hamilton receptor monomer for the stoichiometric molecular imprinting of barbiturates

Lettieri, Stefania, Manesiotis, Panagiotis, Slann, Molly, Lewis, Dewi L, Hall, Andrew J. (2021) A novel Hamilton receptor monomer for the stoichiometric molecular imprinting of barbiturates. Reactive and Functional Polymers, 167 . Article Number 105031. ISSN 1381-5148. (doi:10.1016/j.reactfunctpolym.2021.105031) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:90068)

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Abstract

New molecularly imprinted polymers (MIPs) for the recognition of barbiturates were synthesised by “bulk” polymerisation. These polymers were prepared using pentobarbital as the template in combination with a novel Hamilton receptor functional monomer. The solution binding properties of the monomer were assessed by NMR titration experiments, showing high affinity for barbiturates and lower affinity for related compounds lacking the ability to form as many hydrogen bonds. The properties of the MIP were assessed via equilibrium rebinding experiments and in the chromatographic mode, and compared to the behaviour of a control non-imprinted polymer (NIP). The MIP showed a far higher population of binding sites with higher affinity than the NIP which was reflected in the chromatographic evaluation, where the template and a related barbiturate were not eluted from the MIP within 60 minutes, while their retention was weak on the NIP, leading to extremely high imprinting factors. Other analytes were weakly retained by MIP and NIP, with those presented an acceptor-donor-acceptor array of hydrogen bonding sites most retained. Preliminary molecular modelling studies support the hypothesis that the presence of the template in the MIP synthesis “chooses” the conformation of the functional monomer that is “locked in” during the polymerisation.

Item Type: Article
DOI/Identification number: 10.1016/j.reactfunctpolym.2021.105031
Uncontrolled keywords: molecularly imprinted polymer, barbiturates, Hamilton receptor
Subjects: Q Science > QD Chemistry > QD431 Organic Chemistry- Biochemistry- Proteins, peptides, amino acids
Q Science > QD Chemistry > Analytical Chemistry
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Andrew Hall
Date Deposited: 07 Sep 2021 14:10 UTC
Last Modified: 02 Dec 2021 14:55 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/90068 (The current URI for this page, for reference purposes)
Hall, Andrew J.: https://orcid.org/0000-0001-8849-7063
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