Skip to main content
Kent Academic Repository

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

Biedendieck, Rebekka, Knuuti, Tobias, Moore, Simon J., Jahn, Dieter (2021) The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology. Applied microbiology and biotechnology, . ISSN 1432-0614. E-ISSN 1432-0614. (doi:10.1007/s00253-021-11424-6) (KAR id:89509)

PDF Author's Accepted Manuscript
Language: English

Download this file
[thumbnail of Biedendieck et al.,  2021_unmarked_20210616.pdf]
Request a format suitable for use with assistive technology e.g. a screenreader
XML Word Processing Document (DOCX) Author's Accepted Manuscript
Language: English

Restricted to Repository staff only

Contact us about this Publication
[thumbnail of Biedendieck et al.,  2021_unmarked_20210616.docx]
Official URL:


Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequent systematic knowledge-based development for production purposes. The formation of PHB, a natural product of P. megaterium and potential petro-plastic substitute, is covered and discussed. Further important biotechnological characteristics of P. megaterium for recombinant protein production including high protein secretion capacity and simple cultivation on value-added carbon sources are outlined. This includes the advanced system with almost 30 commercially available expression vectors for the intracellular and extracellular production of recombinant proteins at the g/L scale. We also revealed a novel P. megaterium transcription-translation system as a complementary and versatile biotechnological tool kit. As an impressive biotechnology application, the formation of various cytochrome P450 is also critically highlighted. Finally, whole cellular applications in plant protection are completing the overall picture of P. megaterium as a versatile giant cell factory.

Item Type: Article
DOI/Identification number: 10.1007/s00253-021-11424-6
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Simon Moore
Date Deposited: 29 Jul 2021 06:40 UTC
Last Modified: 14 Nov 2022 23:13 UTC
Resource URI: (The current URI for this page, for reference purposes)

University of Kent Author Information

  • Depositors only (login required):

Total unique views for this document in KAR since July 2020. For more details click on the image.