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Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

Del Rosario, Joanne Marie M., da Costa, Kelly A. S., Asbach, Benedikt, Ferrara, Francesca, Ferrari, Matteo, Wells, David A., Mann, Gurdip Singh, Ameh, Veronica O., Sabeta, Claude T., Banyard, Ashley C., and others. (2021) Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection. Vaccines, 9 (7). p. 741. E-ISSN 2076-393X. (doi:10.3390/vaccines9070741) (KAR id:89035)

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https://dx.doi.org/10.3390/vaccines9070741

Abstract

We developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and Influenza B subtypes (both lineages) to be employed in influenza pseudotype microneutralization (pMN) assays. The pMN is highly sensitive and specific for detecting virus-specific neutralizing antibodies against influenza viruses and can be used to assess antibody functionality in vitro. Here we show the production of these viral HA pseudotypes and their employment as substitutes for wildtype viruses in influenza neutralization assays. We demonstrate their utility in detecting serum responses to vaccination with the ability to evaluate cross-subtype neutralizing responses elicited by specific vaccinating antigens. Our findings may inform further preclinical studies involving immunization dosing regimens in mice and may help in the creation and selection of better antigens for vaccine design. These HA pseudotypes can be harnessed to meet strategic objectives that contribute to the strengthening of global influenza surveillance, expansion of seasonal influenza prevention and control policies, and strengthening pandemic preparedness and response.

Item Type: Article
DOI/Identification number: 10.3390/vaccines9070741
Uncontrolled keywords: influenza; hemagglutinin; pseudotype; vaccine; immunogenicity; monoclonal antibody; neutralization
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 05 Jul 2021 21:04 UTC
Last Modified: 06 Jul 2021 09:26 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/89035 (The current URI for this page, for reference purposes)
Temperton, Nigel J.: https://orcid.org/0000-0002-7978-3815
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