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The Role of Pseudotype Neutralisation Assays in Understanding SARS CoV-2

Cantoni, Diego, Mayora-Neto, Martin, Temperton, Nigel J. (2021) The Role of Pseudotype Neutralisation Assays in Understanding SARS CoV-2. Oxford Open Immunology, . Article Number iqab005. E-ISSN 2633-6960. (doi:10.1093/oxfimm/iqab005) (KAR id:86594)

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Official URL
https://dx.doi.org/10.1093/oxfimm%2Fiqab005

Abstract

Neutralisation assays are crucial tools to quantify the presence of functional neutralising antibodies in serum samples. Since the SARS-CoV-2 virus (the causative agent of COVID-19) is designated as a category 3 biosafety level pathogen, pseudotyped viruses bearing the SARS-CoV-2 spike protein permit extensive and widespread serum/plasma screening in a BSL 2 laboratory. These assays can be used to assess viral tropism, vaccine immunogenicity, efficacy of antiviral compounds (incl. therapeutic mAbs) and serosurveillance studies. In this article, we highlight approaches to SARS-CoV-2 viral pseudotyping, its practicality, and utility in increasing our understanding of neutralising antibodies against SARS-CoV-2.

Item Type: Article
DOI/Identification number: 10.1093/oxfimm/iqab005
Uncontrolled keywords: COVID-19, SARS CoV-2, neutralisation assays
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 14 Feb 2021 16:10 UTC
Last Modified: 16 Feb 2021 14:18 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/86594 (The current URI for this page, for reference purposes)
Temperton, Nigel J.: https://orcid.org/0000-0002-7978-3815
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