Distinct distribution of specific members of protein 4.1 gene family in the mouse nephron

Ramez, Mohamed and Blot-Chabaud, Marcel and Cluzeaud, Françoise and Chanan, Sumita and Patterson, Michael and Walensky, Loren D. and Marfatia, Shirin and Baines, Anthony J. and Chasis, Joel A. and Conboy, John G. and Mohandas, Narla and Gascard, Philippe (2003) Distinct distribution of specific members of protein 4.1 gene family in the mouse nephron. Kidney international, 63 (4). pp. 1321-1337. ISSN 0085-2538. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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BACKGROUND: Protein 4.1 is an adapter protein that links the actin cytoskeleton to various transmembrane proteins. These 4.1 proteins are encoded by four homologous genes, 4.1R, 4.1G, 4.1N, and 4.1B, which undergo complex alternative splicing. Here we performed a detailed characterization of the expression of specific 4.1 proteins in the mouse nephron. METHODS: Distribution of renal 4.1 proteins was investigated by staining of paraformaldehyde-fixed mouse kidney sections with antibodies highly specific for each 4.1 protein. Major 4.1 splice forms, amplified from mouse kidney marathon cDNA, were expressed in transfected COS-7 cells in order to assign species of known exon composition to proteins detected in kidney. RESULTS: A 105 kD 4.1R splice form, initiating at ATG-2 translation initiation site and lacking exon 16, but including exon 17B, was restricted to thick ascending limb of Henle's loop. A 95 kD 4.1N splice form, lacking exons 15 and 17D, was expressed in either descending or ascending thin limb of Henle's loop, distal convoluted tubule, and all regions of the collecting duct system. A major 108 kD 4.1B splice form, initiating at a newly characterized ATG translation initiation site, and lacking exons 15, 17B, and 21, was present only in Bowman's capsule and proximal convoluted tubule (PCT). There was no expression of 4.1G in kidney. CONCLUSION: Distinct distribution of 4.1 proteins along the nephron suggests their involvement in targeting of selected transmembrane proteins in kidney epithelium and, therefore, in regulation of specific kidney functions.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Science Technology and Medical Studies > School of Biosciences > Cell & Developmental Biology Group
Depositing User: Anthony Baines
Date Deposited: 12 Sep 2008 10:27
Last Modified: 15 Apr 2014 16:00
Resource URI: https://kar.kent.ac.uk/id/eprint/8635 (The current URI for this page, for reference purposes)
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