Bennett, Pauline M. and Baines, Anthony J. and Lecomte, Marie-Christine and Maggs, Alison M. and Pinder, Jennifer C. (2004) Not just a plasma-membrane protein - in cardiac muscle cell alpha-II spectrin also shows a close association with myofibrils. Journal of Muscle Research and Cell Motility, 25 (2). pp. 119-126. ISSN 0142-4319. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)
Spectrin and its associated proteins are essential for the integrity of muscle cells and there is increasing evidence for their involvement in signalling pathways as well as having a structural function in mediating stress. Spectrin is a multigene family and it is essential to determine which isoforms are present and their location in the cell. In heart muscle, we have found that one spectrin isoform, alphaII-spectrin, is strongly represented and, using immunofluorescence, we show that it lies within the contractile fibres near the Z-disc as well as on the cardiomyocyte plasma membrane. Electron microscopy of immunogold-labelled cryosections reveals statistically significant clustering of gold particles near the Z-disc, within and close to the edge of myofibrils. betaII-spectrin and ankyrin-R and G are both known to occupy this region. We suggest that alphaIIbetaII spectrin tetramers with ankyrin organise and/or stabilise cardiac muscle cell membrane components relative to the contractile apparatus.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences > Cell & Developmental Biology Group|
|Depositing User:||Anthony Baines|
|Date Deposited:||17 Sep 2008 15:15|
|Last Modified:||29 Apr 2014 15:22|
|Resource URI:||https://kar.kent.ac.uk/id/eprint/8633 (The current URI for this page, for reference purposes)|