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Convalescent plasma therapy for the treatment of patients with COVID‐19: Assessment of methods available for antibody detection and their correlation with neutralising antibody levels

Harvala, Heli, Robb, Matthew L., Watkins, Nick, Ijaz, Samreen, Dicks, Steven, Patel, Monika, Supasa, Piyada, Wanwisa, Dejnirattisai, Liu, Chang, Mongkolsapaya, Juthathip, and others. (2020) Convalescent plasma therapy for the treatment of patients with COVID‐19: Assessment of methods available for antibody detection and their correlation with neutralising antibody levels. Transfusion Medicine, . ISSN 0958-7578. E-ISSN 1365-3148. (doi:10.1111/tme.12746) (KAR id:84860)

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https://dx.doi.org/10.1111/tme.12746

Abstract

Introduction

Blood samples were collected from 52 individuals with a previous laboratory‐confirmed SARS‐CoV‐2 infection. These were assayed for SARS‐CoV‐2 nAbs by microneutralisation and pseudo‐type assays and for antibodies by four different ELISAs. Receiver operating characteristic (ROC) analysis was used to further identify sensitivity and specificity of selected assays to identify samples containing high nAb levels.

Results

Robust associations between nAb titres and reactivity in several ELISA‐based antibody tests demonstrate their possible utility for scaled‐up production of convalescent plasma containing potentially therapeutic levels of anti‐SARS‐CoV‐2 nAbs.

Item Type: Article
DOI/Identification number: 10.1111/tme.12746
Uncontrolled keywords: convalescent plasma, COVID‐19, neutralising antibody level, SARS‐CoV‐2 testing of plasma
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 20 Dec 2020 00:07 UTC
Last Modified: 16 Feb 2021 14:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/84860 (The current URI for this page, for reference purposes)
Temperton, Nigel: https://orcid.org/0000-0002-7978-3815
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