Harvala, Heli, Robb, Matthew L., Watkins, Nick, Ijaz, Samreen, Dicks, Steven, Patel, Monika, Supasa, Piyada, Wanwisa, Dejnirattisai, Liu, Chang, Mongkolsapaya, Juthathip, and others. (2020) Convalescent plasma therapy for the treatment of patients with COVID‐19: Assessment of methods available for antibody detection and their correlation with neutralising antibody levels. Transfusion Medicine, . ISSN 0958-7578. E-ISSN 1365-3148. (doi:10.1111/tme.12746) (KAR id:84860)
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| Official URL https://dx.doi.org/10.1111/tme.12746 |
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Abstract
Introduction
Blood samples were collected from 52 individuals with a previous laboratory‐confirmed SARS‐CoV‐2 infection. These were assayed for SARS‐CoV‐2 nAbs by microneutralisation and pseudo‐type assays and for antibodies by four different ELISAs. Receiver operating characteristic (ROC) analysis was used to further identify sensitivity and specificity of selected assays to identify samples containing high nAb levels.
Results
Robust associations between nAb titres and reactivity in several ELISA‐based antibody tests demonstrate their possible utility for scaled‐up production of convalescent plasma containing potentially therapeutic levels of anti‐SARS‐CoV‐2 nAbs.
| Item Type: | Article |
|---|---|
| DOI/Identification number: | 10.1111/tme.12746 |
| Uncontrolled keywords: | convalescent plasma, COVID‐19, neutralising antibody level, SARS‐CoV‐2 testing of plasma |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
| Depositing User: | Nigel Temperton |
| Date Deposited: | 20 Dec 2020 00:07 UTC |
| Last Modified: | 16 Feb 2021 14:17 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/84860 (The current URI for this page, for reference purposes) |
| Temperton, Nigel: |  https://orcid.org/0000-0002-7978-3815 |
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