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Slow Growth and Increased Spontaneous Mutation Frequency in Respiratory Deficient afo1- Yeast Suppressed by a Dominant Mutation in ATP3

Li, Jing, Rinnerthaler, Mark, Hartl, Johannes, Weber, Manuela, Karl, Thomas, Breitenbach-Koller, Hannelore, Mülleder, Michael, Vowinckel, Jakob, Marx, Hans, Sauer, Michael, and others. (2020) Slow Growth and Increased Spontaneous Mutation Frequency in Respiratory Deficient afo1- Yeast Suppressed by a Dominant Mutation in ATP3. G3: Genes | Genomes | Genetics (G3), 10 (11). Article Number g3.401537. E-ISSN 2160-1836. (doi:10.1534/g3.120.401537) (KAR id:84062)

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https://doi.org/10.1534/g3.120.401537

Abstract

A yeast deletion mutation in the nuclear-encoded gene, AFO1, which codes for a mitochondrial ribosomal protein, led to slow growth on glucose, the inability to grow on glycerol or ethanol, and loss of mitochondrial DNA and respiration. We noticed that afo1- yeast readily obtains secondary mutations that suppress aspects of this phenotype, including its growth defect. We characterized and identified a dominant missense suppressor mutation in the ATP3 gene. Comparing isogenic slowly growing rho-zero and rapidly growing suppressed afo1- strains under carefully controlled fermentation conditions showed that energy charge was not significantly different between strains and was not causal for the observed growth properties. Surprisingly, in a wild-type background, the dominant suppressor allele of ATP3 still allowed respiratory growth but increased the petite frequency. Similarly, a slow-growing respiratory deficient afo1- strain displayed an about twofold increase in spontaneous frequency of point mutations (comparable to the rho-zero strain) while the suppressed strain showed mutation frequency comparable to the repiratory-competent WT strain. We conclude, that phenotypes that result from afo1- are mostly explained by rapidly emerging mutations that compensate for the slow growth that typically follows respiratory deficiency.

Item Type: Article
DOI/Identification number: 10.1534/g3.120.401537
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Campbell Gourlay
Date Deposited: 11 Nov 2020 11:40 UTC
Last Modified: 23 Mar 2021 07:53 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/84062 (The current URI for this page, for reference purposes)
Gourlay, Campbell W.: https://orcid.org/0000-0002-2373-6788
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