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Self-organization of the human embryo in the absence of maternal tissues

Shahbazi, Marta N., Jedrusik, Agnieszka, Vuoristo, Sanna, Recher, Gaelle, Hupalowska, Anna, Bolton, Virginia, Fogarty, Norah M. E., Campbell, Alison, Devito, Liani G., Ilic, Dusko, and others. (2016) Self-organization of the human embryo in the absence of maternal tissues. Nature Cell Biology, 18 (6). pp. 700-708. ISSN 1465-7392. (doi:10.1038/ncb3347) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:84034)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1038/ncb3347

Abstract

Remodelling of the human embryo at implantation is indispensable for successful pregnancy. Yet it has remained mysterious because of the experimental hurdles that beset the study of this developmental phase. Here, we establish an in vitro system to culture human embryos through implantation stages in the absence of maternal tissues and reveal the key events of early human morphogenesis. These include segregation of the pluripotent embryonic and extra-embryonic lineages, and morphogenetic rearrangements leading to generation of a bilaminar disc, formation of a pro-amniotic cavity within the embryonic lineage, appearance of the prospective yolk sac, and trophoblast differentiation. Using human embryos and human pluripotent stem cells, we show that the reorganization of the embryonic lineage is mediated by cellular polarization leading to cavity formation. Together, our results indicate that the critical remodelling events at this stage of human development are embryo-autonomous, highlighting the remarkable and unanticipated self-organizing properties of human embryos.

Item Type: Article
DOI/Identification number: 10.1038/ncb3347
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Alison Campbell
Date Deposited: 10 Nov 2020 12:25 UTC
Last Modified: 10 Nov 2020 12:25 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/84034 (The current URI for this page, for reference purposes)
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