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A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films

Boyd, Hannah, Gonzalez-Martinez, Juan F., Welbourn, Rebecca J.L., Gutfreund, Philipp, Klechikov, Alexey, Robertsson, Carolina, Wickström, Claes, Arnebrant, Thomas, Barker, Robert, Sotres, Javier and others. (2020) A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films. Journal of Colloid and Interface Science, 584 . pp. 660-668. ISSN 1095-7103. E-ISSN 1522-1601. (doi:10.1016/j.jcis.2020.10.124) (KAR id:83792)

Abstract

Hypothesis: Salivary pellicles i.e., thin films formed upon selective adsorption of saliva, protect oral surfaces against chemical and mechanical insults. Pellicles are also excellent aqueous lubricants. It is generally accepted that reconstituted pellicles have a two-layer structure, where the outer layer is mainly composed of MUC5B mucins. We hypothesized that by comparing the effect of ionic strength on reconstituted pellicles and MUC5B films we could gain further insight into the pellicle structure.

Experiments: Salivary pellicles and MUC5B films reconstituted on solid surfaces were investigated at different ionic strengths by Force Spectroscopy, Quartz Crystal Microbalance with Dissipation, Null Ellipsometry and Neutron Reflectometry.

Findings: Our results support the two-layer structure for reconstituted salivary pellicles. The outer layer swelled when ionic strength decreased, indicating a weak polyelectrolyte behavior. While initially the MUC5B films exhibited a similar tendency, this was followed by a drastic collapse indicating an interaction between exposed hydrophobic domains. This suggests that mucins in the pellicle outer layer form complexes with other salivary components that prevent this interaction. Lowering ionic strength below physiological values also led to a partial removal of the pellicle inner layer. Overall, our results highlight the importance that the interactions of mucins with other pellicle components play on their structure.

Item Type: Article
DOI/Identification number: 10.1016/j.jcis.2020.10.124
Uncontrolled keywords: salivary pellicle, mucin, MUC5B, ionic strength, steric forces
Subjects: Q Science > QC Physics > QC176.8.N35 Nanoscience, nanotechnology
Q Science > QD Chemistry > QD473 Physical properties in relation to structure
Q Science > QP Physiology (Living systems) > QP517 Biochemistry
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Signature Themes: Future Human
Funders: Organisations -1 not found.
Depositing User: Robert Barker
Date Deposited: 28 Oct 2020 11:52 UTC
Last Modified: 04 Jul 2023 13:52 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/83792 (The current URI for this page, for reference purposes)

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