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In vitro seed germination and plantlet regeneration of Vanilla siamensis: an endemic species in Thailand

Chaipanich, Vinan V., Roberts, David L., Yenchon, Sureerat, Sompong, Te-chato, Divakaran, Minoo (2020) In vitro seed germination and plantlet regeneration of Vanilla siamensis: an endemic species in Thailand. ScienceAsia, 46 (3). pp. 315-322. E-ISSN 1513-1874. (doi:10.2306/scienceasia1513-1874.2020.040) (KAR id:83579)

Abstract

This study reports the in vitro germination of self-pollinated pod of Vanilla siamensis, native to Thailand. The aim of the present work is to determine the effect of culture media and plant growth regulators on seed germination and plantlet regeneration. The vanilla seeds were asymbiotically germinated on different culture media under aseptic conditions. The results showed that, New Dogashima medium (NDM) supplemented with 2% (w/v) sucrose, 15% (v/v) coconut water (CW) and 0.7% (w/v) agar gave the highest levels of seed germination at 10.1%. The fastest and highest percentage seed germination was achieved using NDM supplemented with 2 mg/l gibberellic acid (GA3). Seeds on this culture medium germinated within 7–8 weeks in comparison with 10–11 weeks on culture medium without plant growth regulators. Protocorms were transferred to different culture media with various concentrations of 6- benzyladenine (BA) to assess the protocorm development. The results revealed that 1⁄2 Murashige and Skoog (1⁄2 MS) medium gave the higher result in survival rate of protocorm than NDM. 1⁄2 MS medium with 0.5 mg/l BA was suitable for shoot formation. Plantlet development was completed after subculturing on 1⁄2 MS medium supplemented with 0.5 mg/l α-naphthaleneacetic acid (NAA) for 8 weeks and successfully acclimatized at 91.7%. The protocol outline here can be used for mass propagation of this endangered species and used as a guideline for improvement of V. siamensis and its in vitro conservation.

Item Type: Article
DOI/Identification number: 10.2306/scienceasia1513-1874.2020.040
Subjects: Q Science > QH Natural history > QH581.2 Cell Biology
Q Science > QH Natural history > QH75 Conservation (Biology)
Q Science > QK Botany
Divisions: Faculties > Social Sciences > School of Anthropology and Conservation
Faculties > Social Sciences > School of Anthropology and Conservation > Biodiversity Conservation Group
Faculties > Social Sciences > School of Anthropology and Conservation > Biodiversity Management Group
Faculties > Social Sciences > School of Anthropology and Conservation > DICE (Durrell Institute of Conservation and Ecology)
Depositing User: David Roberts
Date Deposited: 20 Oct 2020 23:12 UTC
Last Modified: 21 Oct 2020 10:45 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/83579 (The current URI for this page, for reference purposes)
Roberts, David L.: https://orcid.org/0000-0001-6788-2691
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