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Inline holographic microscopy through fiber imaging bundles

Hughes, Michael R (2020) Inline holographic microscopy through fiber imaging bundles. Applied Optics, 60 (4). A1-A7. ISSN 1559-128X. E-ISSN 2155-3165. (doi:10.1364/AO.403805) (KAR id:83241)


Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase conjugation approaches are complex and require extensive calibration. This article describes how simple inline holographic microscopy can be performed through an imaging bundle using a partially coherent illumination source from a multimode fiber. The sample is imaged in transmission, with the intensity hologram sampled by the bundle and transmitted to a remote camera. The hologram can then be numerically refocused for volumetric imaging, achieving a resolution of approximately 6 µm over a depth range of 1 mm. The scheme does not require any complex prior calibration and hence is insensitive to bending.

Item Type: Article
DOI/Identification number: 10.1364/AO.403805
Uncontrolled keywords: fibre imaging, holographic microscopy
Subjects: Q Science > QC Physics > QC355 Optics
Divisions: Divisions > Division of Natural Sciences > Physics and Astronomy
Depositing User: Michael Hughes
Date Deposited: 02 Oct 2020 19:28 UTC
Last Modified: 04 Mar 2024 18:56 UTC
Resource URI: (The current URI for this page, for reference purposes)

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