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Talin Rod Domain Containing Protein 1 (TLNRD1) is a novel actin-bundling protein which promotes filopodia formation

Cowell, Alana R, Jacquemet, Guillaume, Singh, Abhimanyu K., Ammon, York-Christoph, Brown, David G., Akhmanova, Anna, Ivaska, Johanna, Goult, Benjamin T (2020) Talin Rod Domain Containing Protein 1 (TLNRD1) is a novel actin-bundling protein which promotes filopodia formation. BioRxiv, . (doi:10.1101/2020.05.19.103606) (KAR id:81343)

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Talin is a mechanosensitive adapter protein which couples integrins to the cytoskeleton and regulates integrin-mediated adhesion. Talin rod domain-containing protein-1 (TLNRD1) shares 22% homology with the R7R8 domains of talin, and is highly conserved throughout vertebrate evolution, however little is known about its function. Here we show that TLNRD1 is an α-helical protein which shares the same atypical topology as talin R7R8, but forms a novel antiparallel dimer arrangement. Actin co-sedimentation assays and electron microscopy reveal TLNRD1 is an actin-bundling protein that forms tight actin bundles. In addition, TLNRD1 binds to the same LD-motif containing proteins, RIAM and KANK, as talin, and thus may act in competition with talin. Filopodia are cell protrusions supported by tightly bundled actin filaments and TLNRD1 localises to filopodia tips, increases filopodia number and promotes cell migration in 2D. Together our results suggest that TLNRD1 has similar functionality to talin R7R8, serving as a nexus between the actin and microtubule cytoskeletons independent of adhesion complexes.

Item Type: Article
DOI/Identification number: 10.1101/2020.05.19.103606
Uncontrolled keywords: talin, TLNRD1, actin, filopodia, adhesion
Subjects: Q Science > QH Natural history > QH581.2 Cell Biology
Divisions: Divisions > Division of Natural Sciences > Biosciences
Funders: Biotechnology and Biological Sciences Research Council (
International Human Frontier Science Program Organization (
Depositing User: Ben Goult
Date Deposited: 21 May 2020 17:53 UTC
Last Modified: 12 Jul 2022 10:41 UTC
Resource URI: (The current URI for this page, for reference purposes)
Goult, Benjamin T:
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