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Regional Cell-Specific Transcriptome Mapping Reveals Regulatory Complexity in the Adult Drosophila Midgut

Dutta, Devanjali, Dobson, Adam J., Houtz, Philip L., Gläßer, Christine, Revah, Jonathan, Korzelius, Jerome, Patel, Parthive H., Edgar, Bruce A., Buchon, Nicolas (2015) Regional Cell-Specific Transcriptome Mapping Reveals Regulatory Complexity in the Adult Drosophila Midgut. Cell Reports, 12 (2). pp. 346-358. ISSN 2211-1247. (doi:10.1016/j.celrep.2015.06.009) (KAR id:81260)

Abstract

Deciphering contributions of specific cell types to organ function is experimentally challenging. The Drosophila midgut is a dynamic organ with five morphologically and functionally distinct regions (R1–R5), each composed of multipotent intestinal stem cells (ISCs), progenitor enteroblasts (EBs), enteroendocrine cells (EEs), enterocytes (ECs), and visceral muscle (VM). To characterize cellular specialization and regional function in this organ, we generated RNA-sequencing transcriptomes of all five cell types isolated by FACS from each of the five regions, R1–R5. In doing so, we identify transcriptional diversities among cell types and document regional differences within each cell type that define further specialization. We validate cell-specific and regional Gal4 drivers; demonstrate roles for transporter Smvt and transcription factors GATAe, Sna, and Ptx1 in global and regional ISC regulation, and study the transcriptional response of midgut cells upon infection. The resulting transcriptome database (http://flygutseq.buchonlab.com) will foster studies of regionalization, homeostasis, immunity, and cell-cell interactions.

Note: Graphical abstract on paper

Item Type: Article
DOI/Identification number: 10.1016/j.celrep.2015.06.009
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 15 May 2020 13:38 UTC
Last Modified: 16 May 2020 03:12 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/81260 (The current URI for this page, for reference purposes)

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