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Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress

Ogden, Henry B, Fallowfield, Joanne L, Child, Robert B, Davison, Glen, Fleming, Simon C, Edinburgh, Robert M, Delves, Simon K, Millyard, Alison, Westwood, Caroline S, Layden, Joseph D and others. (2020) Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress. Physiological Reports, 8 (5). pp. 1-17. ISSN 2051-817X. (doi:10.14814/phy2.14374) (KAR id:80684)

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Purpose:Exertional heat stress adversely distrupts (GI) barrier integrity and, through subsequent microbial translocation (MT), negativly impacts health. Despite widespread application, the temporal reliability of popular GI barrier integity and MT biomarkers is poorly characterised.

Results: No GI barrier integrity or MT biomarker, except absolute Bacteroides DNA, displayed systematic trial order bias (p ≥ .05). I‐FABP (trial 1 = Δ 0.834 ± 0.445 ng ml−1; trial 2 = Δ 0.776 ± 0.489 ng ml−1) and CLDN‐3 (trial 1 = Δ 0.317 ± 0.586 ng ml−1; trial 2 = Δ 0.371 ± 0.508 ng ml−1) were increased post‐EHST (p ≤ .01). All MT biomarkers were unchanged post‐EHST. Coefficient of variation and typical error of measurement post‐EHST were: 11.5% and 0.004 (ratio) for the DSAT 90‐min postprobe ingestion; 12.2% and 0.004 (ratio) at 150‐min postprobe ingestion; 12.1% and 0.376 ng ml−1 for I‐FABP; 4.9% and 0.342 ng ml−1 for CLDN‐3; 9.2% and 0.420 µg ml−1 for LBP; 9.5% and 0.15 pg µl−1 for total 16S DNA; and 54.7% and 0.032 for Bacteroides/total 16S DNA ratio.

Conclusion: Each GI barrier integrity and MT translocation biomarker, except Bacteroides/total 16S ratio, had acceptable reliability at rest and postexertional heat stress.

Item Type: Article
DOI/Identification number: 10.14814/phy2.14374
Uncontrolled keywords: endotoxin, exercise, gut
Subjects: G Geography. Anthropology. Recreation > GV Recreation. Leisure > Sports sciences
Q Science > QP Physiology (Living systems)
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC1200 Sports medicine
R Medicine > RC Internal medicine > RC1235 Physiology of sports
U Military Science > U Military Science (General)
Divisions: Divisions > Division of Natural Sciences > School of Sport and Exercise Sciences
Depositing User: Glen Davison
Date Deposited: 01 Apr 2020 07:30 UTC
Last Modified: 16 Feb 2021 14:12 UTC
Resource URI: (The current URI for this page, for reference purposes)
Ogden, Henry B:
Fallowfield, Joanne L:
Davison, Glen:
Fleming, Simon C:
Millyard, Alison:
Westwood, Caroline S:
Layden, Joseph D:
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