Skip to main content

Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production

Budge, James D., Knight, Tanya J., Povey, Jane, Roobol, Joanne, Brown, Ian R., Singh, Gurdeep, Dean, Andrew, Turner, Sarah, Jaques, Colin M., Young, Robert J., and others. (2020) Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production. Data in Brief, 29 . p. 105217. ISSN 2352-3409. (doi:10.1016/j.dib.2020.105217) (KAR id:80444)

PDF Publisher pdf
Language: English


Creative Commons Licence
This work is licensed under a Creative Commons Attribution 4.0 International License.
Download (1MB) Preview
[img]
Preview
Official URL
https://doi.org/10.1016/j.dib.2020.105217

Abstract

The data presented in this article relates to the manuscript entitled ‘Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production’, published in the Journal Metabolic Engineering [1]. In the article here, we present data examining the overexpression of the lipid metabolism modifying genes SCD1 and SREBF1 in CHO cells by densitometry of western blots and by using mass spectrometry to investigate the impact on specific lipid species. We also present immunofluorescence data at the protein level upon SCD1 and SREBF1 overexpression. The growth profile data during batch culture of control CHO cells and CHO cells engineered to overexpress SCD1 and SREBF1 during batch culture are also reported. Finally, we report data on the yields of model secretory recombinant proteins produced from control, SCD1 or SREBF1 engineered cells using a transient expression systems.

Item Type: Article
DOI/Identification number: 10.1016/j.dib.2020.105217
Uncontrolled keywords: Chinese hamster ovary (CHO) cells, Lipid metabolism engineering, Cell line engineering, Difficult to express protein, Recombinant protein, Endoplasmic reticulum, Transcription factor engineering
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 10 Mar 2020 10:36 UTC
Last Modified: 10 Mar 2020 10:36 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/80444 (The current URI for this page, for reference purposes)
Smales, Christopher Mark: https://orcid.org/0000-0002-2762-4724
  • Depositors only (login required):

Downloads

Downloads per month over past year