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Automatic motion compensation for structured illumination endomicroscopy using a flexible fiber bundle

Thrapp, Andrew D, Hughes, Michael R. (2020) Automatic motion compensation for structured illumination endomicroscopy using a flexible fiber bundle. Automatic motion compensation for structured illumination endomicroscopy using a flexible fiber bundle, 25 (2). Article Number UNSPECIFIED. ISSN 1083-3668. (doi:10.1117/1.JBO.25.2.026501) (KAR id:80228)

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https://dx.doi.org/10.1117/1.JBO.25.2.026501

Abstract

Significance: Confocal laser scanning enables optical sectioning in clinical fiber bundle endomicroscopes, but lower-cost, simplified endomicroscopes use widefield incoherent illumination instead. Optical sectioning can be introduced in these simple systems using structured illumination microscopy (SIM), a multiframe digital subtraction process. However, SIM results in artifacts when the probe is in motion, making the technique difficult to use in vivo and preventing the use of mosaicking to synthesize a larger effective field of view (FOV).

Aim: We report and validate an automatic motion compensation technique to overcome motion artifacts and allow generation of mosaics in SIM endomicroscopy.

Approach: Motion compensation is achieved using image registration and real-time pattern orientation correction via a digital micromirror device. We quantify the similarity of moving probe reconstructions to those acquired with a stationary probe using the relative mean of the absolute differences (MAD). We further demonstrate mosaicking with a moving probe in mechanical and freehand operation.

Results: Reconstructed SIM images show an improvement in the MAD from 0.85 to 0.13 for lens paper and from 0.27 to 0.12 for bovine tissue. Mosaics also show vastly reduced artifacts.

Conclusion: The reduction in motion artifacts in individual SIM reconstructions leads to mosaics that more faithfully represent the morphology of tissue, giving clinicians a larger effective FOV than the probe itself can provide.

Item Type: Article
DOI/Identification number: 10.1117/1.JBO.25.2.026501
Projects: [UNSPECIFIED] Ultrathin fluorescence microscope in a needle
Subjects: Q Science > QC Physics > QC355 Optics
Divisions: Faculties > Sciences > School of Physical Sciences > Applied Optics Group
Depositing User: A.D. Thrapp
Date Deposited: 25 Feb 2020 12:25 UTC
Last Modified: 04 May 2020 08:43 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/80228 (The current URI for this page, for reference purposes)
Thrapp, Andrew D: https://orcid.org/0000-0003-0777-6218
Hughes, Michael R.: https://orcid.org/0000-0001-5301-2492
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