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Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq

Vito, Davide, Eriksen, Jens Christian, Skjødt, Christian, Weilguny, Dietmar, Rasmussen, Søren K., Smales, C. Mark (2020) Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq. iScience, 23 (1). ISSN 2589-0042. (doi:10.1016/j.isci.2019.100785) (KAR id:79922)

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https://doi.org/10.1016/j.isci.2019.100785

Abstract

How the long non-coding RNA (lncRNA) genome in recombinant protein producing Chinese hamster ovary (CHO) cell lines relates to phenotype is not well described. We therefore defined the CHO cell lncRNA transcriptome from cells grown in controlled miniature bioreactors under fed-batch conditions using RNA-Seq to identify lncRNAs and how the expression of these changes throughout growth and between IgG producers. We identify lncRNAs including Adapt15, linked to ER stress, GAS5, linked to mTOR signaling/growth arrest, and PVT1, linked to Myc expression, which are differentially regulated during fed-batch culture and whose expression correlates to productivity and growth. Changes in (non)-coding RNA expression between the seed train and the equivalent day of fed-batch culture are also reported and compared with existing datasets. Collectively, we present a comprehensive lncRNA CHO cell profiling and identify targets for engineering growth and productivity characteristics of CHO cells.

Item Type: Article
DOI/Identification number: 10.1016/j.isci.2019.100785
Uncontrolled keywords: Biological Sciences, Biotechnology, Transcriptomics
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Mark Smales
Date Deposited: 03 Feb 2020 09:07 UTC
Last Modified: 27 Feb 2020 15:37 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/79922 (The current URI for this page, for reference purposes)
Smales, C. Mark: https://orcid.org/0000-0002-2762-4724
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