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Dynamic and cell-specific DACH1 expression in human neocortical and striatal development

Castiglioni, V., Faedo, A., Onorati, M., Bocchi, V.D., Li, Z., Iennaco, R., Vuono, R., Bulfamante, G.P., Muzio, L., Martino, G., and others. (2019) Dynamic and cell-specific DACH1 expression in human neocortical and striatal development. Cerebral Cortex, 29 (5). pp. 2115-2124. ISSN 1047-3211. (doi:10.1093/cercor/bhy092) (KAR id:79833)

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Abstract

DACH1 is the human homolog of the Drosophila dachshund gene, which is involved in the development of the eye, nervous system, and limbs in the fly. Here, we systematically investigate DACH1 expression patterns during human neurodevelopment, from 5 to 21 postconceptional weeks. By immunodetection analysis, we found that DACH1 is highly expressed in the proliferating neuroprogenitors of the developing cortical ventricular and subventricular regions, while it is absent in the more differentiated cortical plate. Single-cell global transcriptional analysis revealed that DACH1 is specifically enriched in neuroepithelial and ventricular radial glia cells of the developing human neocortex. Moreover, we describe a previously unreported DACH1 expression in the human striatum, in particular in the striatal medium spiny neurons. This finding qualifies DACH1 as a new striatal projection neuron marker, together with PPP1R1B, BCL11B, and EBF1. We finally compared DACH1 expression profile in human and mouse forebrain, where we observed spatio-temporal similarities in its expression pattern thus providing a precise developmental description of DACH1 in the 2 mammalian species.

Item Type: Article
DOI/Identification number: 10.1093/cercor/bhy092
Uncontrolled keywords: centromere protein E; cyclin dependent kinase; dach1 protein; protein; RNA; unclassified drug, animal tissue; Article; corpus striatum; embryo; fetus development; gene expression profiling; glia cell; human; human tissue; immunodetection; immunofluorescence test; immunohistochemistry; in situ hybridization; medium spiny neuron; mouse; neocortex; nerve cell differentiation; nervous system development; neuroepithelium cell; nonhuman; priority journal; protein expression; RNA sequence; stable expression
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Romina Vuono
Date Deposited: 29 Jan 2020 10:21 UTC
Last Modified: 20 Feb 2020 09:55 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/79833 (The current URI for this page, for reference purposes)
Vuono, R.: https://orcid.org/0000-0002-5162-3422
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