Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

Vasilopoulou, Elisavet (2019) Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy. In: Gnudi, Luigi and Long, David A., eds. Diabetic Nephropathy: Methods and Protocols. Methods in Molecular Biology . Springer. ISBN 978-1-4939-9840-1. (doi:10.1007/978-1-4939-9841-8_5) (KAR id:79288)

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Official URL: http://dx.doi.org/10.1007/978-1-4939-9841-8_5

Abstract

Diabetic nephropathy is associated with injury and loss of podocytes, specialized epithelial cells that are critical for glomerular filtration. This chapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

Item Type:	Book section
DOI/Identification number:	10.1007/978-1-4939-9841-8_5
Uncontrolled keywords:	Diabetes; Diabetic nephropathy; Glomerulus; Kidney; Podocyte
Subjects:	Q Science Q Science > QP Physiology (Living systems)
Divisions:	Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User:	Elisavet Vasilopoulou
Date Deposited:	13 Dec 2019 17:11 UTC
Last Modified:	14 Nov 2022 23:10 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/79288 (The current URI for this page, for reference purposes)

University of Kent Author Information

Vasilopoulou, Elisavet.

Creator's ORCID:	https://orcid.org/0000-0002-1940-0333
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