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Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

Vasilopoulou, Elisavet (2019) Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy. In: Gnudi, Luigi and Long, David A., eds. Diabetic Nephropathy: Methods and Protocols. Methods in Molecular Biology . Springer. ISBN 978-1-4939-9840-1. (doi:10.1007/978-1-4939-9841-8_5) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1007/978-1-4939-9841-8_5

Abstract

Diabetic nephropathy is associated with injury and loss of podocytes, specialized epithelial cells that are critical for glomerular filtration. This chapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.

Item Type: Book section
DOI/Identification number: 10.1007/978-1-4939-9841-8_5
Uncontrolled keywords: Diabetes; Diabetic nephropathy; Glomerulus; Kidney; Podocyte
Subjects: Q Science
Q Science > QP Physiology (Living systems)
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: E. Vasilopoulou
Date Deposited: 13 Dec 2019 17:11 UTC
Last Modified: 16 Dec 2019 16:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/79288 (The current URI for this page, for reference purposes)
Vasilopoulou, Elisavet: https://orcid.org/0000-0002-1940-0333
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