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Developmental control of nuclear size and shape by kugelkern and kurzkern

Brandt, A., Papagiannouli, F, Wagner, N., Wilsch-Bräuninger, M., Braun, M., Furlong, E.E., Loserth, S., Wenzl, C., Pilot, F., Vogt, N., and others. (2006) Developmental control of nuclear size and shape by kugelkern and kurzkern. Current Biology, 16 (6). pp. 543-552. ISSN 0960-9822. (doi:10.1016/j.cub.2006.01.051) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:78995)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/j.cub.2006.01.051

Abstract

Background: The shape of a nucleus depends on the nuclear lamina, which is tightly associated with the inner nuclear membrane and on the interaction with the cytoskeleton. However, the mechanism connecting the differentiation state of a cell to the shape changes of its nucleus are not well understood. We investigated this question in early Drosophila embryos, where the nuclear shape changes from spherical to ellipsoidal together with a 2.5-fold increase in nuclear length during cellularization. Results: We identified two genes, kugelkern and kurzkern, required for nuclear elongation. In kugelkern- and kurzkern-depleted embryos, the nuclei reach only half the length of the wild-type nuclei at the end of cellularization. The reduced nuclear size affects chromocenter formation as marked by Heterochromatin protein 1 and expression of a specific set of genes, including early zygotic genes. kugelkern contains a putative coiled-coil domain in the N-terminal half of the protein, a nuclear localization signal (NLS), and a C-terminal CxxM-motif. The carboxyterminal CxxM motif is required for the targeting of Kugelkern to the inner nuclear membrane, where it colocalizes with lamins. Depending on the farnesylation motif, expression of kugelkern in Drosophila embryos or Xenopus cells induces overproliferation of nuclear membrane. Conclusions: Kugelkern is so far the first nuclear protein, except for lamins, that contains a farnesylation site. Our findings suggest that Kugelkern is a rate-determining factor for nuclear size increase. We propose that association of farnesylated Kugelkern with the inner nuclear membrane induces expansion of nuclear surface area, allowing nuclear growth.

Item Type: Article
DOI/Identification number: 10.1016/j.cub.2006.01.051
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Curr. Biol. [Field not mapped to EPrints] C2 - 16458513 [Field not mapped to EPrints] AD - Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany [Field not mapped to EPrints] AD - Biozentrum, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany [Field not mapped to EPrints] AD - Max-Planck-Institut für Molekulare Zellbiologie und Genetik, Pfotenhauerstr. 108, 01307 Dresden, Germany [Field not mapped to EPrints] AD - European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany [Field not mapped to EPrints] AD - Institut de Biologie du Développement de Marseille (IBDM), Laboratoire de Génétique et de Physiologie du Développement (LGPD), Campus de Luminy, case 907, 13288 Marseille Cedex 09, France [Field not mapped to EPrints] AD - Max-Planck-Institut für Entwicklungsbiologie, Spemannstr. 35, 72076 Tübingen, Germany [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] M3 - Article [Field not mapped to EPrints]
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Fani Papagiannouli
Date Deposited: 26 Nov 2019 15:19 UTC
Last Modified: 26 Nov 2019 15:20 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/78995 (The current URI for this page, for reference purposes)
Papagiannouli, F: https://orcid.org/0000-0003-4094-6688
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