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Stage-specific control of niche positioning and integrity in the Drosophila testis

Schardt, L., Ander, J.-J., Lohmann, I., Papagiannouli, F (2015) Stage-specific control of niche positioning and integrity in the Drosophila testis. Mechanisms of Development, 138 . pp. 336-348. ISSN 0925-4773. (doi:10.1016/j.mod.2015.07.009) (KAR id:78986)

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http://dx.doi.org/10.1016/j.mod.2015.07.009

Abstract

A fundamental question is how complex structures are maintained after their initial specification. Stem cells reside in a specialized microenvironment, called niche, which provides essential signals controlling stem cell behavior. We addressed this question by studying the Drosophila male stem cell niche, called the hub. Once specified, the hub cells need to maintain their position and architectural integrity through embryonic, larval and pupal stages of testis organogenesis and during adult life. The Hox gene Abd-. B, in addition to its described role in male embryonic gonads, maintains the architecture and positioning of the larval hub from the germline by affecting integrin localization in the neighboring somatic cyst cells. We find that the AbdB-Boss/Sev cascade affects integrin independent of Talin, while genetic interactions depict integrin as the central downstream player in this system. Focal adhesion and integrin-adaptor proteins within the somatic stem cells and cyst cells, such as Paxillin, Pinch and Vav, also contribute to proper hub integrity and positioning. During adult stages, hub positioning is controlled by Abd-B activity in the outer acto-myosin sheath, while Abd-B expression in adult spermatocytes exerts no effect on hub positioning and integrin localization. Our data point at a cell- and stage-specific function of Abd-B and suggest that the occurrence of new cell types and cell interactions in the course of testis organogenesis made it necessary to adapt the whole system by reusing the same players for male stem cell niche positioning and integrity in an alternative manner. © 2015 .

Item Type: Article
DOI/Identification number: 10.1016/j.mod.2015.07.009
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Mech. Dev. [Field not mapped to EPrints] C2 - 26226434 [Field not mapped to EPrints] AD - Centre for Organismal Studies (COS) Heidelberg, Cell Networks - Cluster of Excellence, University of HeidelbergD-69120, Germany [Field not mapped to EPrints] AD - Deutsches Krebsforschungszentrum (DKFZ)D-69120, Germany [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints] M3 - Article [Field not mapped to EPrints]
Uncontrolled keywords: Abd-B, Drosophila testis, Hox genes, Integrin, Niche positioning, Talin, adaptor protein, integrin, myosin adenosine triphosphatase, paxillin, talin, Vav protein, Abd-B proteins, Drosophila, Drosophila protein, homeodomain protein, integrin, talin, Abd B gene, adult, animal cell, animal tissue, Article, cell interaction, controlled study, focal adhesion, gene expression, gene interaction, germline stem cell, Hox gene, larval stage, male, nonhuman, organogenesis, priority journal, protein function, protein localization, somatic cell, spermatocyte, stem cell niche, testis development, animal, antagonists and inhibitors, cell adhesion, cell lineage, cytology, Drosophila melanogaster, gene, gene expression regulation, gene silencing, genetics, growth, development and aging, larva, metabolism, morphogenesis, physiology, signal transduction, stem cell niche, testis, transgenic animal, Animals, Animals, Genetically Modified, Body Patterning, Cell Adhesion, Cell Lineage, Drosophila melanogaster, Drosophila Proteins, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genes, Insect, Homeodomain Proteins, Integrins, Larva, Male, Signal Transduction, Stem Cell Niche, Talin, Testis
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Fani Papagiannouli
Date Deposited: 26 Nov 2019 16:02 UTC
Last Modified: 16 Feb 2021 14:10 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/78986 (The current URI for this page, for reference purposes)
Papagiannouli, F: https://orcid.org/0000-0003-4094-6688
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