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Simple liquid chromatographic method for the determination of cefotaxime in human and rat plasma

Ling, S., Yuen, K.H., Barker, S.A. (2003) Simple liquid chromatographic method for the determination of cefotaxime in human and rat plasma. Journal of Chromatography B: Biomedical Sciences and Applications, 783 (1). pp. 297-301. ISSN 1387-2273. (doi:10.1016/S1570-0232(02)00657-8) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:78867)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1016/S1570-0232(02)00657-8

Abstract

A high-performance liquid chromatographic method with ultraviolet (UV) detection was developed for measuring cefotaxime in rat and human plasma. The method used direct injection of the plasma supernatant after deproteinization with 70% perchloric acid. Degradation of cefotaxime in acidic medium was retarded by adding phosphate buffer before centrifuging the sample. The mobile phase was 0.05 M aqueous ammonium acetate–acetonitrile–tetrahydrofuran (87:11:2, v/v) adjusted to pH 5.5. Analysis was run at a flow-rate of 1.0 ml/min, and a detection wavelength of 254 nm was used. The method has a quantification limit of 0.20 μg/ml. The within- and between-day coefficients of variation and accuracy values were less than 8% and ±3%, respectively, while the recovery values were greater than 87% over the concentration range tested (0.20–50 μg/ml). The speed, sensitivity, specificity and reproducibility of this method make it particularly suitable for the routine determination of cefotaxime in human plasma. Moreover, only a relatively small sample plasma volume (100 μl) is required, allowing this method to be applied to samples taken from neonates.

Item Type: Article
DOI/Identification number: 10.1016/S1570-0232(02)00657-8
Uncontrolled keywords: Cefotaxime, Biodegradation, High performance liquid chromatography, Phosphates, Plasma applications, Ultraviolet detectors, Detection wavelength, Biomedical engineering, acetonitrile, ammonium acetate, cefotaxime, perchloric acid, phosphate, tetrahydrofuran, cefotaxime, accuracy, analytic method, article, calibration, controlled study, drug blood level, drug degradation, drug determination, flow rate, high performance liquid chromatography, human, nonhuman, pH, plasma volume, priority journal, rat, reproducibility, animal, blood, methodology, sensitivity and specificity, ultraviolet spectrophotometry, Animals, Cefotaxime, Chromatography, High Pressure Liquid, Humans, Rats, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Susan Barker
Date Deposited: 27 Nov 2019 13:05 UTC
Last Modified: 27 Nov 2019 13:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/78867 (The current URI for this page, for reference purposes)
Barker, S.A.: https://orcid.org/0000-0003-4880-0253
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