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Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin

Hellerschmied, Doris, Lehner, Anita, Franicevic, Nina, Arnese, Renato, Johnson, Chloe, Vogel, Antonia, Meinhart, Anton, Kurzbauer, Robert, Deszcz, Luiza, Gazda, Linn, and others. (2019) Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin. Nature Communications, 10 (1). p. 4781. ISSN 2041-1723. (doi:10.1038/s41467-019-12667-8) (KAR id:77825)

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Myosin is a motor protein that is essential for a variety of processes ranging from intracellular transport to muscle contraction. Folding and assembly of myosin relies on a specific chaperone, UNC-45. To address its substrate-targeting mechanism, we reconstitute the interplay between Caenorhabditis elegans UNC-45 and muscle myosin MHC-B in insect cells. In addition to providing a cellular chaperone assay, the established system enabled us to produce large amounts of functional muscle myosin, as evidenced by a biochemical and structural characterization, and to directly monitor substrate binding to UNC-45. Data from in vitro and cellular chaperone assays, together with crystal structures of binding-deficient UNC-45 mutants, highlight the importance of utilizing a flexible myosin-binding domain. This so-called UCS domain can adopt discrete conformations to efficiently bind and fold substrate. Moreover, our data uncover the molecular basis of temperature-sensitive UNC-45 mutations underlying one of the most prominent motility defects in C. elegans.

Item Type: Article
DOI/Identification number: 10.1038/s41467-019-12667-8
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Michael Geeves
Date Deposited: 25 Oct 2019 12:53 UTC
Last Modified: 25 Oct 2019 12:54 UTC
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