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Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin

Hellerschmied, Doris, Lehner, Anita, Franicevic, Nina, Arnese, Renato, Johnson, Chloe, Vogel, Antonia, Meinhart, Anton, Kurzbauer, Robert, Deszcz, Luiza, Gazda, Linn, and others. (2019) Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin. Nature Communications, 10 (1). Article Number 4781. ISSN 2041-1723. (doi:10.1038/s41467-019-12667-8) (KAR id:77825)

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https://doi.org/10.1038/s41467-019-12667-8

Abstract

Myosin is a motor protein that is essential for a variety of processes ranging from intracellular transport to muscle contraction. Folding and assembly of myosin relies on a specific chaperone, UNC-45. To address its substrate-targeting mechanism, we reconstitute the interplay between Caenorhabditis elegans UNC-45 and muscle myosin MHC-B in insect cells. In addition to providing a cellular chaperone assay, the established system enabled us to produce large amounts of functional muscle myosin, as evidenced by a biochemical and structural characterization, and to directly monitor substrate binding to UNC-45. Data from in vitro and cellular chaperone assays, together with crystal structures of binding-deficient UNC-45 mutants, highlight the importance of utilizing a flexible myosin-binding domain. This so-called UCS domain can adopt discrete conformations to efficiently bind and fold substrate. Moreover, our data uncover the molecular basis of temperature-sensitive UNC-45 mutations underlying one of the most prominent motility defects in C. elegans.

Item Type: Article
DOI/Identification number: 10.1038/s41467-019-12667-8
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Michael Geeves
Date Deposited: 25 Oct 2019 12:53 UTC
Last Modified: 22 Sep 2020 16:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/77825 (The current URI for this page, for reference purposes)
Johnson, Chloe: https://orcid.org/0000-0003-0856-5041
Geeves, Michael: https://orcid.org/0000-0002-9364-8898
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