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High Resolution Respirometry in Candida albicans

Duvenage, Lucian, Munro, Carol, Gourlay, Campbell W. (2019) High Resolution Respirometry in Candida albicans. Bio-protocol, 9 (17). ISSN 2331-8325. (doi:10.21769/BioProtoc.3361)

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https://doi.org/10.21769/BioProtoc.3361

Abstract

Many Candida species, such as the opportunistic human pathogen Candida albicans, are Crabtree-Negative yeasts and are therefore highly dependent on the energy generated through oxidative phosphorylation. Respiration control is linked to a range of aspects of C. albicans cell physiology that appear to be important for virulence, most notably its ability to switch from yeast to hyphal forms and the maintenance of the cell wall. The following protocol allows for the measurement and characterisation of respiration in C. albicans using high resolution respirometry. We outline how addition of respiration inhibitors can be used to assay the “mode” of respiration, mitochondrial health and the level of electron transport that is coupled to ATP synthase activity in living cell cultures. These data provide useful insight into the effects of external factors, such as exposure to anti-fungal compounds, or internal changes such as genetic alterations on respiratory performance.

Item Type: Article
DOI/Identification number: 10.21769/BioProtoc.3361
Uncontrolled keywords: Candida, Respiration, Mitochondria, Respirometry, Yeast, Metabolism
Subjects: Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Campbell Gourlay
Date Deposited: 05 Sep 2019 21:43 UTC
Last Modified: 06 Sep 2019 12:14 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/76219 (The current URI for this page, for reference purposes)
Gourlay, Campbell W.: https://orcid.org/0000-0002-2373-6788
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