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Force-dependent regulation of KANK1-talin complex at focal adhesions

Yu, Miao, Le, Shimin, Ammon, York-Christoph, Goult, Benjamin T, Akhmanova, Anna, Yan, Jie (2019) Force-dependent regulation of KANK1-talin complex at focal adhesions. Nano Letters, . pp. 5982-5990. ISSN 1530-6992. (doi:10.1021/acs.nanolett.9b01732) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:75755)

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KANK proteins mediate cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix. KANKs interact with the integrin/actin-binding protein talin and with several components of microtubule-stabilizing cortical complexes. Due to actomyosin contractility, the talin-KANK complex is likely under mechanical force, and its mechanical stability is expected to be a critical determinant of KANK recruitment to focal adhesions. Here, we quantified the lifetime of the complex of the talin rod domain R7 and the KN domain of KANK1 under shear-force geometry and found that it can withstand forces for seconds to minutes over a physiological force range up to 10 pN. Complex stability measurements combined with cell biological experiments suggest that shear-force stretching promotes KANK1 localization to the periphery of focal adhesions. These results indicate that the talin-KANK1 complex is mechanically strong, enabling it to support the cross-talk between microtubule and actin cytoskeleton at focal adhesions.

Item Type: Article
DOI/Identification number: 10.1021/acs.nanolett.9b01732
Uncontrolled keywords: talin, integrin, kank, adhesion, mechanobiology
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 10 Aug 2019 17:11 UTC
Last Modified: 06 Feb 2020 10:32 UTC
Resource URI: (The current URI for this page, for reference purposes)
Goult, Benjamin T:
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