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Off–on switchable fluorescent probe for prompt and cost-efficient detection of bacteria

Giovannini, Giorgia, Gubala, Vladimir, Hall, Andrew J. (2019) Off–on switchable fluorescent probe for prompt and cost-efficient detection of bacteria. New Journal of Chemistry, 43 (13094). p. 13102. ISSN 1144-0546. (doi:10.1039/C9NJ03110C) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:75704)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1039/C9NJ03110C

Abstract

The rapid and straightforward detection of bacteria in food and human samples is becoming important, particularly in view of the development of point-of-care devices and lab-on-a-chip tools for prevention and treatment of bacterial infections. In the present work, we present the design of a cost-efficient, switchable, fluoresce-based probe for the rapid detection of glucosidase-producing bacteria. The probe was prepared by functionalising streptavidin-coated magnetic particles with a switchable substrate capable of indicating the presence of the metabolic enzyme glucosidase (Glu-C-MPs). Under normal conditions the probe is ‘off’ but is switched ‘on’ in the presence of glucosidase-producing bacteria, leading to a measurable increase in fluorescence, indicating the presence of bacteria in the sample. The selectivity of the probe was firstly evaluated using enzymatic experiments, showing that it can detect both α- and β-glucosidase. Subsequently, we tested the efficacy of the probe in detecting bacteria using microorganisms that tend to infect the urinary tract, inducing over-active bladder (OAB) in women. The probe successfully detected the presence of E. coli, K. pneumoniae and P. aeruginosa at low concentrations (75 CFU), within 60 minutes and with a good signal-to-noise ratio. 3 hours were required to observe an increase in fluorescence in the presence of Enterococcus and S. aureus, indicating that these microorganisms produce lower amounts of the detected enzyme. Glu-C-MPs are a promising tool for the prompt, easy and cost-efficient detection of microorganisms, demonstrating a high selectivity and sensitivity for glucosidase-producing bacteria. Thanks to the simple readout and the low-cost instrumentation required for the analysis, the proposed approach could pave the way for the development of affordable point-of-care devices.

Item Type: Article
DOI/Identification number: 10.1039/C9NJ03110C
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 07 Aug 2019 14:23 UTC
Last Modified: 16 Sep 2020 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/75704 (The current URI for this page, for reference purposes)
Gubala, Vladimir: https://orcid.org/0000-0001-6301-3632
Hall, Andrew J.: https://orcid.org/0000-0001-8849-7063
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