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Measuring the density of DNA films using ultraviolet-visible interferometry

Śmiałek, Małgorzata A., Jones, Nykola C., Hoffmann, Søren Vrønning, Mason, Nigel (2013) Measuring the density of DNA films using ultraviolet-visible interferometry. Physical Review E, 87 (6). ISSN 2470-0053. (doi:10.1103/PhysRevE.87.060701) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1103/PhysRevE.87.060701

Abstract

In order to determine a proper value for the density of dry DNA films we have used a method based upon the measurement of interference effects in transmission spectra of thin DNA layers. Our results show that the methodology is effective and the density of DNA in this state, 1.407 g/cm3, is much lower than the commonly used 1.7 g/cm3. Obtaining accurate values for the DNA film density will allow the optical constants for DNA to be recalculated, which were previously obtained assuming a higher DNA density. Furthermore, since our recent investigations have shown a strong dependence of the sample composition on DNA film formation and thus on its density, such a method will be important in characterizing particle interactions with DNA film and their dose dependence.

Item Type: Article
DOI/Identification number: 10.1103/PhysRevE.87.060701
Uncontrolled keywords: DNA density, DNA films, DNA layers, Dose dependences, Interference effects, Sample composition, Strong dependences, Transmission spectrums, Condensed matter physics, Physics, DNA, artificial membrane, DNA, algorithm, artificial membrane, chemistry, densitometry, interferometry, materials testing, procedures, refractometry, ultrastructure, ultraviolet radiation, Algorithms, Densitometry, DNA, Interferometry, Materials Testing, Membranes, Artificial, Refractometry, Ultraviolet Rays
Subjects: Q Science > QC Physics
Divisions: Faculties > Sciences > School of Physical Sciences
Depositing User: Nigel Mason
Date Deposited: 16 Jul 2019 13:17 UTC
Last Modified: 17 Jul 2019 10:21 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/74719 (The current URI for this page, for reference purposes)
Mason, Nigel: https://orcid.org/0000-0002-4468-8324
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