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Characterization of PAH/DPPG layer-by-layer films by VUV spectroscopy

Duarte, A.A., Gomes, P.J., Ribeiro, J.H.F., Ribeiro, P.A., Hoffmann, S.V., Mason, Nigel, Oliveira Jr., O.N., Raposo, M. (2013) Characterization of PAH/DPPG layer-by-layer films by VUV spectroscopy. European Physical Journal E, 36 (9). (doi:10.1140/epje/i2013-13098-7) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:74716)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
https://doi.org/10.1140/epje/i2013-13098-7

Abstract

The spectroscopic characterization of layer-by-layer (LbL) films containing liposomes is essential not only for determining the precise film architecture but also to guide the design of drug delivery systems. In this study we provide the first report of vacuum ultraviolet spectroscopy (VUV) characterization of LbL films made with liposomes from 1.2-dipalmitoyl-sn-Glycero-3-[Phospho-rac-(1- glycerol)] (Sodium Salt) (DPPG) alternated with poly(allylamine hydrochloride) (PAH). Measurements in the 6.0-9.5eV range allowed us to identify the electronic transitions responsible for the spectra, which were assigned to carboxyl, hydroxyl and phosphate groups in DPPG while the PAH spectra were governed by electronic transitions in the amino groups. The surface mass density of the LbL films could be determined, from which the formation of a DPPG bilayer was inferred. This rupture of the liposomes into bilayers was confirmed with atomic force microscopy measurements. In subsidiary experiments we ensured that the UV irradiation in vacuum had negligible damage in the DPPG liposomes during the course of the VUV measurements. In addition to demonstrating the usefulness of VUV spectroscopy, the results presented here may be exploited in biological applications of liposome-containing films.

Item Type: Article
DOI/Identification number: 10.1140/epje/i2013-13098-7
Uncontrolled keywords: Biological applications, Drug delivery system, Electronic transition, Layer-by-layer films, Poly(allylamine hydrochloride), Spectroscopic characterization, Vacuum ultraviolet spectroscopy, VUV spectroscopy, Biological applications, Drug delivery system, Electronic transition, Layer-by-layer films, Poly(allylamine hydrochloride), Spectroscopic characterization, Vacuum ultraviolet spectroscopy, VUV spectroscopy, Atomic force microscopy, Drug delivery, Ultraviolet spectroscopy, Atomic force microscopy, Characterization, Irradiation, Ultraviolet spectroscopy, Liposomes, Liposomes, 1,2 dipalmitoylphosphatidylglycerol, 1,2-dipalmitoylphosphatidylglycerol, liposome, phosphatidylglycerol, polyallylamine, polyamine, lipid bilayer, liposome, article, chemistry, lipid bilayer, vacuum, X ray photoelectron spectroscopy, chemistry, lipid bilayer, Lipid Bilayers, Liposomes, Phosphatidylglycerols, Photoelectron Spectroscopy, Polyamines, Vacuum, Lipid Bilayers, Liposomes, Phosphatidylglycerols, Photoelectron Spectroscopy, Polyamines, Vacuum
Divisions: Faculties > Sciences > School of Physical Sciences
Depositing User: Nigel Mason
Date Deposited: 16 Jul 2019 10:25 UTC
Last Modified: 16 Jul 2019 10:25 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/74716 (The current URI for this page, for reference purposes)
Mason, Nigel: https://orcid.org/0000-0002-4468-8324
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