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Monoterpene separation by coupling proton transfer reaction time-of-flight mass spectrometry with fastGC

Materić, D., Lanza, M., Sulzer, P., Herbig, J., Bruhn, D., Turner, C., Mason, Nigel, Gauci, V. (2015) Monoterpene separation by coupling proton transfer reaction time-of-flight mass spectrometry with fastGC. Analytical and Bioanalytical Chemistry, 407 (25). pp. 7757-7763. ISSN 1618-2642. (doi:10.1007/s00216-015-8942-5) (KAR id:74679)

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https://doi.org/10.1007/s00216-015-8942-5

Abstract

Proton transfer reaction mass spectrometry (PTR-MS) is a well-established technique for real-time analysis of volatile organic compounds (VOCs). Although it is extremely sensitive (with sensitivities of up to 4500 cps/ppbv, limits of detection <1 pptv and the response times of approximately 100 ms), the selectivity of PTR-MS is still somewhat limited, as isomers cannot be separated. Recently, selectivity-enhancing measures, such as manipulation of drift tube parameters (reduced electric field strength) and using primary ions other than H3O(+), such as NO(+) and O2 (+), have been introduced. However, monoterpenes, which belong to the most important plant VOCs, still cannot be distinguished so more traditional technologies, such as gas chromatography mass spectrometry (GC-MS), have to be utilised. GC-MS is very time consuming (up to 1 h) and cannot be used for real-time analysis. Here, we introduce a sensitive, near-to-real-time method for plant monoterpene research-PTR-MS coupled with fastGC. We successfully separated and identified six of the most abundant monoterpenes in plant studies (α- and β-pinenes, limonene, 3-carene, camphene and myrcene) in less than 80 s, using both standards and conifer branch enclosures (Norway spruce, Scots pine and black pine). Five monoterpenes usually present in Norway spruce samples with a high abundance were separated even when the compound concentrations were diluted to 20 ppbv. Thus, fastGC-PTR-ToF-MS was shown to be an adequate one-instrument solution for plant monoterpene research.

Item Type: Article
DOI/Identification number: 10.1007/s00216-015-8942-5
Uncontrolled keywords: FastGC, Monoterpenes, Pinene, Plant VOCs, PTR-MS, VOC, 3-carene, alkene, beta-pinene, bicyclo compound, myrcene, proton, terpene, volatile organic compound, chemistry, isolation and purification, mass fragmentography, pine, procedures, spruce, volatilization, Alkenes, Bicyclo Compounds, Gas Chromatography-Mass Spectrometry, Monoterpenes, Picea, Pinus, Protons, Volatile Organic Compounds, Volatilization
Divisions: Faculties > Sciences > School of Physical Sciences
Depositing User: Nigel Mason
Date Deposited: 03 Jul 2019 11:56 UTC
Last Modified: 03 Jul 2019 11:57 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/74679 (The current URI for this page, for reference purposes)
Mason, Nigel: https://orcid.org/0000-0002-4468-8324
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