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Development of a DNA Biodosimeter for UV radiation

Marques, T.S., Pires, F., Magalhães-Mota, G., Ribeiro, P.A., Raposo, M., Mason, Nigel (2018) Development of a DNA Biodosimeter for UV radiation. In: Proceedings of the 6th International Conference on Photonics, Optics and Laser Technology. 1. pp. 328-333. SciTePress ISBN 978-989-758-286-8. (doi:10.5220/0006732003280333) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:74654)

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Ultraviolet (UV) radiation has a strong influence in the damage of deoxyribonucleic acid (DNA). In this work, the possibility of a DNA UV radiation dosimeter is evaluated. For that, calf thymus DNA samples, thin films and aqueous solutions, were irradiated with 254 nm wavelength light during different periods of time, being the damage caused by the irradiation analysed by both UV-visible and infrared spectroscopies. As the DNA is a polyelectrolyte, the pH of the DNA samples was also considered as a variable. Results demonstrated that damage in DNA takes place in both thin films and solutions when irradiated at 254 nm, as revealed by a consistent decay in measured absorbance values. However, DNA solutions were seen to give more reliable as the induced damage is easily measured. For this case, the absorbance at 260 nm was seen to exponentially decrease with the irradiation time as a result of radiation damage with the kinetics damage strongly dependent of pH. Consequently, the lifetime of such dosimeter device can be chosen by changing the pH of aqueous solutions.

Item Type: Conference or workshop item (Proceeding)
DOI/Identification number: 10.5220/0006732003280333
Uncontrolled keywords: Biodosimeters, DNA Calf Thymus, UV Radiation, DNA, Dosimeters, Irradiation, Photonics, Polyelectrolytes, Radiation damage, Solutions, Thin films, Thorium alloys, Thymus, Ultraviolet radiation, Absorbance values, Biodosimeters, Calf thymus, Calf thymus DNA, Dosimeter devices, Induced damage, Irradiation time, Radiation dosimeters, Nucleic acids
Divisions: Divisions > Division of Natural Sciences > School of Physical Sciences
Depositing User: Nigel Mason
Date Deposited: 28 Jun 2019 14:49 UTC
Last Modified: 16 Feb 2021 14:05 UTC
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Mason, Nigel:
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